Paneth cells and stomach

From:Gayle Callis

My brand new Wheators Functional Histology text,Churchill Livingston
publishers,with a super CD, shows paneth cells a being found at the base of
crypts (of Lieberkuhn), and the text states they are distinguished by
eosinophilic apical, prominent granules.  They show a good H&E stain, also
an phloxine tartrazine stain with the latter being quite spectacular! 

As for the stomach, this book also has an excellent diagram of a stomach,
looking very much the same as a mouse stomach.  We had to look at the area
near the entrance to small intestine, duodenum, and dissected the stomach
out, bisected and oriented accordingly, on edge, and the same for every
stomach.  If precise area must always be located, a green marking ink is
helpful. You can always fill a stomach with fixative, after rinsing out
contents, tie off each end to keep stomach distended with NBF fixative.
This improves fixation, then gross tissue afterwards for careful paraffin
processing and easier orientation, same can be done with gut, or just
perfuse gut with fixative, then immerse for longer fixation. 

Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology - Marsh Lab
Montana State University - Bozeman
19th and Lincoln St
Bozeman MT 59717-3610

406 994-6367 (lab with voice mail)
406 994-4303 (FAX)


<< Previous Message | Next Message >>