From:"Molinari, Betsy"


Does anyone have an ORO staining protocol on non embedded mouse aorta? In the procedure the mice hearts are perfused with a desired fixative for a determined amount of time. Then the aorta is dissected out and stained with ORO while the aorta is intact. After staining, the aorta is then bisected and photographed. No processing or embedding is used. I have found several abstracts but most are vague about the staining procedure. Has anyone had an experience with this technique?

   I believe I told a histonetter that I would forward a Movats protocol to them but I lost the email... If this is still needed, please contact me and I will send it.

Betsy Molinari  HT  ASCP

Texas Heart Institute

Houston, TX 77373


832-355-6812 (fax)

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