Nitro Blue Tetrazolium or NADH

From:Kathleen Cormier

Hello All,

We are looking to stain some tissues with NBT. The pathologist that I work 
with has a theory that the non specific staining that we see in the liver 
and muscle in our NBF and "special IHC Fixatives" ( our special fixatives 
for IHC are a continuing saga don't ask) slides my be related to 
mitochondria. I have searched Histonet and various other sources and come 
up with a NADH-diaphorase protoccol. But it only seems to work on snap 
frozen tissue. Any suggestions? Comments? References? Protocols? Thanks!

Histology Supervisor

<< Previous Message | Next Message >>