Nitro Blue Tetrazolium or NADH
Hello All,
We are looking to stain some tissues with NBT. The pathologist that I work
with has a theory that the non specific staining that we see in the liver
and muscle in our NBF and "special IHC Fixatives" ( our special fixatives
for IHC are a continuing saga don't ask) slides my be related to
mitochondria. I have searched Histonet and various other sources and come
up with a NADH-diaphorase protoccol. But it only seems to work on snap
frozen tissue. Any suggestions? Comments? References? Protocols? Thanks!
Kathy
Histology Supervisor
Divisi
<< Previous Message | Next Message >>