Masson's Trichrome (heating of Bouin's fixative in microwave)

From:Merkey Marjorie

Good Afternoon:
We are aware that some procedures offer quick mordanting/time savings by
heating Bouin's in the microwave and are seeking more information from labs
who have been doing this routinely. We are concerned about the picric acid
explosions hazard. If you heat Bouin's in the microwave, we would like to
know the safe temperature range, possible time/power setting, and the amount
of time sections should sit in heated Bouin's for mordanting before
continuing with the stain.
Marjorie Merkey
Clinical Support Manager
HCA Richmond Hospital Laboratories
office: 804-327-4075   pager: 759-6942

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