Dear Histonetters,
I'm trying to do a TRAP stain on bone tissue with light green as the 
counterstain.  The color product for the TRAP stain cannot be cleared 
to xylene, because it will dissolve, so I have been trying to aqueous 
mount the slides.  However, I am finding that the light green is 
fading very fast (within a week).  I was wondering if light green is 
not compatible with aqueous mounting?  Do sections stained with light 
green have to mounted with a xylene-based medium?
thanks in advance for any assistance,   John
-- 
John A. Baker
The University of Michigan
Orthopaedic Research Laboratories
Histology Unit
400 North Ingalls, G160
Ann Arbor, MI 48109-0486
Main lab office phone:734-763-9674
Histology office:734-936-1635