Here's a message Gail sent to me instead of the list. Please reply to
her directly as I'm not sure she is recieving Histonet messages yet.
Thanks


Is there a known protocol for immunhistochemical staining of adsorbed
ECM 
from, say, fibroblasts? I've trawled the online literature but the only
ECM 
analysis I've ever been able to find is via SDS-PAGE (electrophoresis)
or 
chromatography.

There appear to be loads of papers where the cells have been lysed and

subsequent antibody staining of collagen/fibronectin etc done - but can

this actually be described as 'extracellular' matrix since the cells
have 
to be entered to stain for it?

Here's hoping :)

Thanks,

Gail (Donegan)

Department of Clinical Engineering,
The University of Liverpool,
Duncan Building,
Daulby Street,
Liverpool,
L69 3GA.
U.K.