IHC of ECM
Here's a message Gail sent to me instead of the list. Please reply to
her directly as I'm not sure she is recieving Histonet messages yet.
Is there a known protocol for immunhistochemical staining of adsorbed
from, say, fibroblasts? I've trawled the online literature but the only
analysis I've ever been able to find is via SDS-PAGE (electrophoresis)
There appear to be loads of papers where the cells have been lysed and
subsequent antibody staining of collagen/fibronectin etc done - but can
this actually be described as 'extracellular' matrix since the cells
to be entered to stain for it?
Here's hoping :)
Department of Clinical Engineering,
The University of Liverpool,
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