Embryo Processing

From:Keith Rogers

Dear Histonetters,

We routinely process mouse embryos from day 7dpc and up. We have a request 
to process embryos that have been pre-stained with an alkaline phosphate 
substrate - INT BCIP. This substrate is alcohol soluble. Routine paraffin 
processing, no matter how short, reduces or eliminates the staining. Can 
anyone suggest alternative dehydrants that I can use? There are some 
methods that use highly toxic solvents, we would like to avoid these is 

Many thanks,

Keith Rogers
SAIC Frederick

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