Cryosections of insects

From:LINDA MARGRAF MD

Here's a message Alice was having trouble sending to the list.


Dear Collegues,

at the time I am working on a methode for cryosections on small insects

e.g. aphids or collembola. because we have no machine to embed the
whole 
insects in paraffine this technique is accompanied with a high loss of

specimen and a long retention period (approx. 1 h) in high temerpature

(60 degr. Celsius) paraffine. 
Does anyone of you know a reference for a cryosection methode with
insects 
of this size?

thank you in advance,
AliceDipl. Biol. Alice B. Czarnetzki
Institut fuer Agraroekologie
F.A.L. Braunschweig
Bundesallee 50
38116 Braunschweig
Germany

Phone: 0049 (0) 531 5962526
alice.czarnetzki@fal.de 
http://www.fal.de/tebbe 




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