Hi everybody,

I wonder whether there have been done any refinements on the "old" 
methods of muscle fiber typing for their contractile and metabolic 
character (e.g. Brooke and Kaiser, 1970). As far as I know there is no 
combined staining method that enables me to differentiate 4 fiber types 
as the molecular genetic methods do. Furthermore I'm looking for 
software that allows to a) (semi)automatically detect the fiber types 
and b) to calculate area and diameter of the fibers.

I appreciate any advice!

Daniel Moerlein
Institut for Animal Breeding
Martin-Luther-University
Halle
Germany