mouse perfusion- LFB
Thanks to you all for your advice. I think the damage in my tissue
happens even before I put the sections in the LFB solution- so it is
probably a matter of poor dehydration (I hope). I'm going to try
fixing a brain in formalin, without perfusion, and then dehydrate in
30% sucrose for 24h. Does this make sense?
Also, for the people who have trouble with sections floating off the
slide: try Superfrost Plus Gold slides, they work pretty well.
Brigham & Women's Hospital
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