I'm nissl staining thin (35-50 micron) mouse brain sections, and having
problems with the sections folding around the edges during the staining
procedure. They look fine when I slice them (on a cryostat) and mount
them on gelatin-subbed slides, but after processing they're folded and
some sections have come off the slides completely. I've noticed that
this happens more often in the EtOH steps than xylene steps, for what
that's worth. Any advice would be great. Thanks

mark