Thanks for the reply. I have been quite busy so I have not had a
chance to be in touch. The pieces of bone I am working with are
about 1cm across and 5mm thick. The bone is attached to a
tendon which is also difficult to section. I have decided to process
the samples overnight using my usual 24hr cycle for soft tissues.
I will just extend the paraffin infiltration which is at 56-58C.
What is clearite 3 and propar? I have never used these.
I would like to know what your favourite haematoxylin is for
staining bone. Also, do you have any tips for working with very
tough tendon . What adhesive do you use on your slides? I use Aptes
but I am not sure if there is a better one for bone. I still have
slight lifting. Gosh, sorry about all the questions. I love and
hate working with bone samples. I have had fairly good slides but
they are not as perfect as I would like them to be and would
therefore appreciate some expert advice.
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