bone IHC

From:"Coskran, Timothy M"


Does anyone have any recommendations on how to keep bone sections on your
slides through antigen retrieval steps involving heat?  We have tried most
slide types and drying procedures that I'm aware of.  We have shown that the
immunoreactivity we are looking for works best with steam citrate retrieval.
However, the bones are the tissues of interest and we can never get past the
point of retrieval.  All tissues are negative without retrieval.  We may
move into frozens, but are trying to salvage the formalin fixed paraffin
embedded tissue we have.

Tim Coskran

Discovery Pathology

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