Judy Trogadis wrote:

> Dear Histonettors:
>
> I am trying to cut sections of rat lung tissue, about 200 microns thick, with a vibratome. It is difficult, to say the least, because the lung was inflated just prior to fixation and, although well fixed, retained a spongy consistency and therefore not firm enough to section. I have embedded it in agar without any improvement.
>
> Does anyone have any suggestions.
> Thank you
>
> Judy
>

Vacuum embedding should give better infiltration and eliminate the spongy areas. If the lung was not fixed in a vacuum, the spongy areas may be poorly fixed.

Geoff
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff@umdnj.edu
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