Re: mouse perfusion
What exactly is not working with the LFB? What is your staining protocol?
Veterinary Clinical Sciences
Ohio State University
----- Original Message -----
From: "Christina Benou"
Sent: Friday, August 17, 2001 12:01 PM
Subject: mouse perfusion
> Hello to all,
> I am new to histopathology and I've been trying for a long time now
> to get the Luxol fast blue stain for mouse brain working. I work on
> frozen sections. I have heard that the best way to reveal myelin
> pathology is to sac and immediately perfuse the mouse with
> paraformaldehyde 4%. Is that right? Would anyone share a protocol
> about this? any help is appreciated!
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