Re: mouse perfusion

From:Nancy E Weber

What exactly is not working with the LFB?  What is your staining protocol?

Nancy Weber
Research Associate
Veterinary Clinical Sciences
Ohio State University
----- Original Message ----- 
From: "Christina Benou" 
Sent: Friday, August 17, 2001 12:01 PM
Subject: mouse perfusion

> Hello to all,
> I am new to histopathology and I've been trying for a long time now 
> to get the Luxol fast blue stain for mouse brain working. I work on 
> frozen sections. I have heard that the best way to reveal myelin 
> pathology is to sac and immediately perfuse the mouse with 
> paraformaldehyde 4%. Is that right? Would anyone share a protocol 
> about this? any help is appreciated!
> Thanks
> Christina

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