Re: bone IHC


Hi there,
 I recently had a great deal of trouble with this situation,
I found that drying the slides flat in the oven for up to a week made a big
Most of my sections were coming off while cooling down from a full immersion
citrate retrieval at 85C ( in water bath), I found that by allowing the
colution to cool quickly by adding citrate iceblocks, was the best answer,
now i lose very few sections.

Case Sinclair
Adelaide Centre for Spinal Research
Frome Road
SA  5001
----- Original Message -----
From: "Coskran, Timothy M" 
To: "Histonet (E-mail)" 
Sent: Saturday, August 25, 2001 12:54 AM
Subject: bone IHC

> Hello,
> Does anyone have any recommendations on how to keep bone sections on your
> slides through antigen retrieval steps involving heat?  We have tried most
> slide types and drying procedures that I'm aware of.  We have shown that
> immunoreactivity we are looking for works best with steam citrate
> However, the bones are the tissues of interest and we can never get past
> point of retrieval.  All tissues are negative without retrieval.  We may
> move into frozens, but are trying to salvage the formalin fixed paraffin
> embedded tissue we have.
> Thanks,
> Tim Coskran
> Discovery Pathology
> Pfizer
> 860-715-4558
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