Re: antibody for T Cells on mouse tissues

From:"Savage, Tony TJ"

For Jennifer Harvey,

		The antibody we routinely use for T cells in formalin fixed
mouse tissue is the DAKO anti-human CD3 antibody. We use this primary after
the tissues have received  20 minutes microwaving in pH6 citrate buffer.
Good luck

			Tony Savage

	Tony Savage
	Histopathology Group
          Respiratory, Inflammation & Respiratory Pathogens CEDD	
         GlaxoSmithKline Medicines Research Centre,
	Gunnelswood Road,
	      Herts.	   SG1  2NY
	  United Kingdom

Tel.       +44  01438  764117
Fax.      +44  01438  764488
mobile  +44 07753609835

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