Re: Preserving specimens for LCM

From:richard hazelton

Dear Liz,

Once the cells have been captured they are relatively stable on the caps, so
it may be better to save caps rather than sections. Caps may be stored
at -70C, -20Cor even 4C for a couple of weeks.
If your sections are fixed before storage, morphology may be better
preserved, but for RNA, the best results are obtained when the specimen is
processed without delay. A cryopreservative such as sucrose/glycerol may be
satisfactory, but I haven't tried to recover RNA from sections stored this
way. This gives good results for IHC but RNA is very labile in an aqueous

-----Original Message-----
From: Liz Lummus 
To: Histonet 
Date: Saturday, 18 August 2001 7:52
Subject: Preserving specimens for LCM

>I am currently using LCM to extract RNA from mouse
>testis. The results are good using freshly cut
>specimens on glass slides. I would like to store extra
>slide samples at -70C for future use, but the
>morphology after thawing makes it difficult to pick
>out cell structure due to ice artifact. Is there any
>way of storing the samples that will preserve both the
>RNA and the morphology, or am I asking too much?
>Thanks in advance,
>Liz Lummus
>UTSWMC @ Dallas
>Do You Yahoo!?
>Make international calls for as low as $.04/minute with Yahoo! Messenger

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