Re: Churukian Silver stain for Argyophil granules
Hi Karen,
I was doing Churukian's somewhat frequently up until about 6 mo ago. You
need to hydrate section to acidified water, ph 4.2 with 0.3% Citric Acid
instead of plain Distilled Water. You need to make up the Bodian's
Developer fresh. And having a microwave would be a great help. You need
some source of heat, or oven, in any case. I've got a real good protocol
if you need it, let me know.
Nancy Weber
Research Associate
Veterinary Clinical Sciences
Ohio State University
----- Original Message -----
From: WIGHTMAN_KAREN_A@LILLY.COM
To: histonet@pathology.swmed.edu
Sent: Friday, August 31, 2001 9:13 AM
Subject: Churukian Silver stain for Argyophil granules
Fellow Histonetters
Has anyone done a Churukian Silver lately? I would appreciate any special
hints or tips since it's been a long, long time since I've done any. Thanks
very much
Karen Wightman
Eli Lilly & Co
Greenfield, IN46140
315-277-4136
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