Hi,
    Using DAKO CD117 (aka Ckit) with Envision+ (polyclonal) we get great
results at 1:200 for 30 min. with no pretreatment. Use a good piece of colon
for a control, there are usually loads of mast cells there.
Amos Brooks
----- Original Message -----
From: "E Sharon Shields" 
To: 
Sent: Wednesday, August 22, 2001 1:22 PM
Subject: CD117


I have been asked to develope CD117 as a marker for gastrointestional
tumors.  Any suggestion on the best  clone and vendor to use?
Thank you
E.S. Shields
Baptist Hospital of E TN