Hi Cindy,
I haven't had that problem with BVD, however I did see it with an 
antibody to metallothionein. In my case though, the slides had been 
cut for months, not days. I fixed the problem by storing my slides 
(paraffin sections, air-dried only) in a slide box in a -20C freezer, for 
long-term storage. Maybe your climate, (hot, and more humid) has 
hastened the process I was experiencing here on the East coast.
Just a thought.
Cheers! Greg

Date sent:      	Mon, 27 Aug 2001 10:18:48 -0500
From:           	Cindy Chard-Bergstrom 
Subject:        	BVD staining
Forwarded to:   	DOBBIN@acad1.cs.upei.ca
To:             	histonet@pathology.swmed.edu

> I am experiencing problems with our BVD (bovine viral diarrhea) stain. My controls are precut, sometimes a week in advance. Slides are not heated until right before they are stained. At first the control stains very nice, but over time the staining on subsequent runs is diminished. Only a few 
cells will stain. I make up the antibody dilution fresh each time I use it. Is anyone else doing this stain and if so have  you experienced this problem? Any suggestions, anyone?
> 
> 
> 
> Cindy Chard-Bergstrom B.S.,HT(ASCP),QIHC
> Microbiologist III 
> Kansas State University
> chardb@vet.ksu.edu
> 
>