My processing stations are all set at 40 minutes from 10% BNF to the 3rd
histoclear, then 45 min for each paraffin (3 changes). I have no problems.
Also, how do you fix your tissues? What are you fixing tissues in? We
allow our tissues to fix in 10% BNF for 3-4 days before trimming in and
At 12:25 AM 1/26/70 -0500, April E. Monchik wrote:
>I am a technician in a research institution core facility. My
>question pertains to paraffin sectioning.
>When I cut rat heart tissue it looks like the paraffin has not
>infiltrated. There are many holes in the tissue. This happens even
>when I use a knew knife.
> I use a Tissue Tek to embed the tissues. The tissue go into the
>alcohol for several changes of thirty minutes each. The histoclear
>steps are thirty minutes each too. The paraffin changes are 30 mins,
>1 hour, and 30 mins.
>Does anyone have any suggestions for embedding rat heart and spleen
>tissue? I'm interested in a protocol that really allows for
>infiltration into the tissues. Thanks for your help
Veterinary Diagnostics Lab
Utah State University
fax (435) 797-2805
<< Previous Message | Next Message >>