My processing stations are all set at 40 minutes from 10% BNF to the 3rd
histoclear, then 45 min for each paraffin (3 changes).  I have no problems.
 Also, how do you fix your tissues?  What are you fixing tissues in? We
allow our tissues to fix in 10% BNF for 3-4 days before trimming in and
processing.  

Connie McManus


At 12:25 AM 1/26/70 -0500, April E. Monchik wrote:
>I am a technician in a research institution core facility.  My 
>question pertains to paraffin sectioning.
>When I cut rat heart tissue it looks like the paraffin has not 
>infiltrated.  There are many holes in the tissue.  This happens even 
>when I use a knew knife.
>   I use a Tissue Tek to embed the tissues.  The tissue go into the 
>alcohol for several changes of thirty minutes each.  The histoclear 
>steps are thirty minutes each too.  The paraffin changes are 30 mins, 
>1 hour, and 30 mins.
>Does anyone have any suggestions for embedding rat heart  and spleen 
>tissue?  I'm interested in a protocol that really allows for 
>infiltration into the tissues.   Thanks for your help
>
>

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