How long are you vacuuming the tissue in last paraffin?
University of CA, San Diego
Department of Orthopedics
La Jolla, CA
"April E. Monchik" wrote:
> I am a technician in a research institution core facility. My
> question pertains to paraffin sectioning.
> When I cut rat heart tissue it looks like the paraffin has not
> infiltrated. There are many holes in the tissue. This happens even
> when I use a knew knife.
> I use a Tissue Tek to embed the tissues. The tissue go into the
> alcohol for several changes of thirty minutes each. The histoclear
> steps are thirty minutes each too. The paraffin changes are 30 mins,
> 1 hour, and 30 mins.
> Does anyone have any suggestions for embedding rat heart and spleen
> tissue? I'm interested in a protocol that really allows for
> infiltration into the tissues. Thanks for your help
<< Previous Message | Next Message >>