I is my experience that ZincFormalin(not to be confused with Zn fix without
formalin) can require pre-treatment if left in the fixative too long.  I
guess the formalin in the fixative eventually does cross-link?


                                                                                    
                    "Morken, Tim"                                                   
                                        cc:     "'eva.alstromer@histolab.se'"      
                                                         
                    08/31/01             Subject:     RE: Zinkformalin              
                    01:35 PM                                                        
                                                                                    
                                                                                    




Eva, You will not need any antigen retrieval (enzyme or head -mediated) if
you are using a zinc fixative.

Tim Morken
CDC, Atlanta
eva.alstromer@histolab.se
-----Original Message-----
From: Eva Alstr#246#mer [mailto:eva.alstromer@histolab.se]
Sent: Friday, August 31, 2001 7:43 AM
To: histonet@pathology.swmed.edu
Subject: Zinkformalin



Dear histonetters,

I have a question about Zink-(acetate)formalin, do you need to use antigen
retrieval before staining
immunohistochemistry?
Thanks, in advance
Eva Alstromer, Sweden