Teri,
I've done a similar stain on adult mice. The reference is Tarpley,
Biotechnic & Histochemistry, vol 74, no. 3 p 116-118. Instead of alizarin
red I used murexide. Send me your mailing address off list and I can send
you a reprint if you don't have access to the journal and the color plates.

John E. Tarpley 5-1-A
Associate Scientist
Amgen Inc.
One Amgen Center Drive
Thousand Oaks, CA 91320 

-----Original Message-----
From: Johnson, Theresa [mailto:TJJ@Stowers-Institute.org]
Sent: Thursday, August 23, 2001 7:27 AM
To: Histonet (E-mail)
Subject: Alcian blue and Alizarin red on mouse skeletons


Hi all!

I've obtained a procedure for performing Alcian blue and Alizarin red
staining on mouse embryo skeletons (thanks Robbin Newlin).  However, one of
our investigators wants to do this procedure on an older mouse (days to
weeks old).  Can anybody who has done this procedure give me any information
regarding its feasibility or chances of success?

Or, since this is research, you just try it and see? 

Teri Johnson
Lab Manager, Histology
Stowers Institute of Medical Research
Kansas City, Missouri