Preserving specimens for LCM

From:Liz Lummus

I am currently using LCM to extract RNA from mouse
testis. The results are good using freshly cut
specimens on glass slides. I would like to store extra
slide samples at -70C for future use, but the
morphology after thawing makes it difficult to pick
out cell structure due to ice artifact. Is there any
way of storing the samples that will preserve both the
RNA and the morphology, or am I asking too much? 

Thanks in advance,

Liz Lummus
Biochemistry
UTSWMC @ Dallas

lizlummus@yahoo.com  

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