P-JNK IHC on brain sections

From:Fulvio Magara

Dear colleagues,
I unsuccessfully tried several times to get an immunostaining for 
phosphorylated JNK (Phospho-SAPK) , using an antibody from New England 
BioLab (now Cell SIgnalling) on mouse brain sections.
No staining at all on paraffin sections, even after 10 incubation in 95°C 
citrate buffer; nor on frozen sections (where I had occasional staining of 
hippocampal mossy fibers)
Has anybody experience with this antibody on brain sections?

Fulvio Magara, Ph.D.
Institut de Biologie Cellulaire et Morphologie
Université de Lausanne
Rue du Bugnon, 9
CH - 1005 Lausanne

Tel. +41 +21 692 51 53 Office
	          692 51 05 FAX
	   +31 372 87 27 Home

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