Eyes - lenses
Hello - I have a question for all of you who work with eyes. We are
doing a study with canine eyes and cataracts. We have to keep the
orientation of the lens in the eye during processing. The eyes have been
cut in half and the half with the lens is processed and embedded. The
problem we have been running into with some (not all) of the eyes is that
during processing the lens moves. Any ideas on how to keep it
stationary? As the eyes are half width, we use deep processing
cassettes. The researcher does not want to cut the eyes any thinner.
Also, they are presently fixing the eyes in 10% NBF and we are
processing rather much like Anne described yesterday. Now the researcher
wants to use Zenker's. I have vetoed this and have suggested Davidson's
which we have used with success in other eye projects. Any opinions on
fixatives you like? Tried and true doesn't seem to be the order for
some. The researcher wants better initial penetration and something that
will harden the eye and keep the lens from cracking.
Any ideas or opinions will be appreciated.
Cheryl Crowder, BA, HTL(ASCP)
Department of Pathobiological Sciences
School of Veterinary Medicine
Louisiana State University
Baton Rouge, LA 70803
FAX (225) 578-9720
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