Eyes - lenses

From:Cheryl Crowder

Hello - I have a question for all of you who work with eyes.  We are 
doing a study with canine eyes and cataracts.  We have to keep the 
orientation of the lens in the eye during processing.  The eyes have been 
cut in half and the half with the lens is processed and embedded.  The 
problem we have been running into with some (not all) of the eyes is that 
during processing the lens moves.  Any ideas on how to keep it 
stationary?  As the eyes are half width, we use deep processing 
cassettes.  The researcher does not want to cut the eyes any thinner.  
     Also, they are presently fixing the eyes in 10% NBF and we are 
processing rather much like Anne described yesterday.  Now the researcher 
wants to use Zenker's.  I have vetoed this and have suggested Davidson's 
which we have used with success in other eye projects.  Any opinions on 
fixatives you like?  Tried and true doesn't seem to be the order for 
some.  The researcher wants better initial penetration and something that 
will harden the eye and keep the lens from cracking.
     Any ideas or opinions will be appreciated.

Cheryl Crowder, BA, HTL(ASCP)
Chief Technologist
Department of Pathobiological Sciences
School of Veterinary Medicine
Louisiana State University
Baton Rouge, LA  70803

(225) 578-9734
FAX  (225) 578-9720

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