Carstairs for fibrin
Fellow Histonetters,
I need to do the Carstair's method for fibrin written in Sheehan's
book.
The protocol lists making and using 20 ml
aqueous saturated picric acid in addition to
making and using 80 ml of picric acid in saturated
isopropanol. What is the purpose of the saturated picric
acid in the isopropanol? Doesn't that mean we need to have the
picric acid crystals? Can the orange-G be made in the
saturated aqueous solution and still
work?
One other question, the procedure lists formalin-saline as the
fixative. Is this the preferred fixative or does the stain work
just as well on tissues fixed in 10% NBF.
Thanks in advance,
Cathy
*********************************
GLP Compliant Laboratory
Cathy A. Mayton
Project Director
Wasatch Histo Consultants, Inc.
www.wasatchhisto.com
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