Carstairs for fibrin

From:Cathy Mayton

Fellow Histonetters,

I need to do the Carstair's method for fibrin written in Sheehan's book.

The protocol lists making and using 20 ml aqueous saturated picric acid in addition to making and using 80 ml of picric acid in saturated isopropanol.  What is the purpose of the saturated picric acid in the isopropanol?  Doesn't that mean we need to have the picric acid crystals?  Can the orange-G be made in the saturated aqueous solution and still work?

One other question, the procedure lists formalin-saline as the fixative.  Is this the preferred fixative or does the stain work just as well on tissues fixed in 10% NBF.

Thanks in advance,

GLP Compliant Laboratory

Cathy A. Mayton
Project Director
Wasatch Histo Consultants, Inc.

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