CD 99 from DAKO

From:=?iso-8859-1?q?Steve=20Machin=20UK?=

Hi,
Can anyone help us with a problem with our CD99 from Dako?
We use 1:1000 1hr and get lots of background and weak staining.
With microwave we get almost no background and almost no staining.
We use peroxide blocker but no protein blocker because we only need
that with polyclonals.  Dako CD99 is monoclonal mouse.

Best Wishes
Steve Machin UK

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