BCIP/NBT insolubility


 A research worker is finding that the BCIP/NBT end product she is 
using on her insitu whole mount preparations (13.5dpc mouse 
embryo's) is "leaching" out in the alcohols (despite only a few 
minutes in each ethanol) on processing to paraffin wax. She is 
looking at hair cells in the cochlea and although some colour is 
retained, it is not strong enough for photography/publication.
Does anyone know of a method for stabilizing this end product 
(which I thought was insoluble) or getting p/w sections without 
alcohol dehydration (e.g. DMSO).Morphology is critical here so no 
frozen sections and preferably not a water based resin!
I await your replies with the same optimism I have that England will 
demolish the Germans (at football) this Saturday!
Many thanks,
Terry Hacker,
Head of Histopathology and Electron Microscopy
Medical Research Council,
Oxfordshire, OX11 ORD
01235 824521
Fax 01235 834776
e-mail t.hacker@har.mrc.ac.uk

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