From:"April E. Monchik"

I am a technician in a research institution core facility.  My 
question pertains to paraffin sectioning.
When I cut rat heart tissue it looks like the paraffin has not 
infiltrated.  There are many holes in the tissue.  This happens even 
when I use a knew knife.
   I use a Tissue Tek to embed the tissues.  The tissue go into the 
alcohol for several changes of thirty minutes each.  The histoclear 
steps are thirty minutes each too.  The paraffin changes are 30 mins, 
1 hour, and 30 mins.
Does anyone have any suggestions for embedding rat heart  and spleen 
tissue?  I'm interested in a protocol that really allows for 
infiltration into the tissues.   Thanks for your help




<< Previous Message | Next Message >>