I am a technician in a research institution core facility. My
question pertains to paraffin sectioning.
When I cut rat heart tissue it looks like the paraffin has not
infiltrated. There are many holes in the tissue. This happens even
when I use a knew knife.
I use a Tissue Tek to embed the tissues. The tissue go into the
alcohol for several changes of thirty minutes each. The histoclear
steps are thirty minutes each too. The paraffin changes are 30 mins,
1 hour, and 30 mins.
Does anyone have any suggestions for embedding rat heart and spleen
tissue? I'm interested in a protocol that really allows for
infiltration into the tissues. Thanks for your help
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