zinc formalin fixation
| From: | Cheryl Clarke <2boxers00@home.com> |
Just an inquiry to anyone in Histoland,....Could someone please give me some
information on zinc formalin fixation? Can you substitute this for B5
fixation? Does it have any bad side effects on gene rearrangement? We have
just started a trial of zinc formalin and so far the IMP staining is good,
but am interested in feedback from longtime users. Thanks in advance, will
wait for your opinions.
C Clarke
____________________________________________________________________________
______________
-----Original Message-----
From: HistoNet Server [mailto:histonet@pathology.swmed.edu]
Sent: Monday, August 06, 2001 10:38 PM
To: HistoNet Server
Subject: Daily Digest
----------------------------------------------------------------------
Date: 6 Aug 2001 00:27:46 -0500
From: "David Taylor Manager" <DTMan@kingmower.com.au>
Subject: RE: Re processing Tiny Biopsies, esp. paste like material
Dear Steve, This is a good tip. We use this technique, (steps 6,7 and 8)
when embedding curettes and POC's etc, but with foam pads. David.
David Taylor
Laboratory Manager
Drs King & Mower
Adelaide, Australia
- -----Original Message-----
From: Steve Machin UK [mailto:stevemachinuk@yahoo.co.uk]
Sent: Sunday, 5 August 2001 22:56
To: Histonet Histonet Histonet
Subject: Re processing Tiny Biopsies, esp. paste like material
I have a trick to share with you which enables us to process tiny
fragments of tissue too small to pick up.
CellPath sell biowrap paper which remains stiff after processing and
will hold tiny fragments of material, e.g. bone marrow aspirates.
The paper is made for wrapping small samples and I'm sure other
people sell similar material.
1. Cut the end off a plastic dropping pipette.
2. Suck up the tissue fragments.
3. SLOWLY drop the sample onto the biowrap paper which is placed onto
a dowmflow bench or a wad of absorbant material. The excess fluid
will be drawn away and leave the sample in a small spot. The slower
you do this the smaller the spot will be.
4. Wrap up the paper and process.
5. At embedding, unwrap the paper and make sure the wax on it remains
molten.
6. Keep a mold hot and put in the paper specimen-side-down.
7. Keep the mold hot until the wax is melted both above and below the
paper, add a little wax to the mold at this stage.
8. This is the hard bit. When melted, quickly transfer the mould and
paper to the cold plate and press the paper into the mould with one
finger. AS SOON as the wax begins to set, peel the paper off the
mould and, hey presto, all the tiny bits of tissue are stuck to the
mould and not the paper.
9. Add more wax to the mould and remelt. Allow the bits to settle to
the bottom on the mould and then set the block ready for sectioning.
10. Make sure the microtome chuck is perfectly aligned or you you
will trim away the sample. For a tip on aligning the mircotome chuck
see my homepage on histonet.
Best Wishes
Steve Machin UK
____________________________________________________________
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Get your free @yahoo.co.uk address at http://mail.yahoo.co.uk
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----------------------------------------------------------------------
Date: 6 Aug 2001 04:21:34 -0500
From: "Anthony f. Brandwood" <afbrand@liverpool.ac.uk>
Subject: Re: textbooks
- --On 06 August 2001 16:12 +1200 Aidan Schurr <Aidan.Schurr@hvh.co.nz>
wrote:
> Hi all,
>
> I'm preparing a "wish-list" for the year. Can anyone recommend a good,
> new textbook (a "must-have" if you will). I have a copy of the latest
> edition of Bancroft already. I tried to source a new edition of Sheehan
> and Hrapchak, but it appears this has been out of print since 1987!
>
> Thanks for all your comments in advance.
>
> Cheers,
> Aidan
Dear Aidan,
You can get a copy of 'Theory and Practice of Histotechnology,2nd Edition'
by Sheehan and Hrapchak from ThermoShandon.It is on page 96 of their latest
catalogue. It says that the text is a Shandon exclusive!
Best Wishes,
Tony Brandwood,
Dept. of Veterinary Pathology,
University of Liverpool,
P.O.Box 147,
Liverpool,
L69 3BX.
Tel. No. 0151 794 4251
Fax. No. 0151 794 4268
>
>
> __
>
> aidan schurr b.m.l.sc
> section head, histology
> hutt valley district health board
> lower hutt
> new zealand
>
> aidan.schurr@hvh.co.nz
> ++64 4 570 9173
>
>
----------------------------------------------------------------------
Date: 6 Aug 2001 04:43:06 -0500
From: "Neuropathology" <Neuropath.Frenchay@dial.pipex.com>
Subject: Re: Re processing Tiny Biopsies, esp. paste like material
We use the Shandon Biopsy Bags for small bits, pastes etc. To get the
tissue in we stick the bag over the base of a powder funnel and pour the
liquid and contents through the funnel. If there is a residue in the pot we
just add water and pour through again. This method is very good when you
have lots of small fragments mixed up with blood. We use powder funnels
because the stem has a wider bore.
If we only have a very few tiny pieces then we hand process.
Andy Shand
- ----- Original Message -----
From: Steve Machin UK <stevemachinuk@yahoo.co.uk>
To: Histonet Histonet Histonet <histonet@pathology.swmed.edu>
Sent: Sunday, August 05, 2001 2:26 PM
Subject: Re processing Tiny Biopsies, esp. paste like material
> I have a trick to share with you which enables us to process tiny
> fragments of tissue too small to pick up.
> CellPath sell biowrap paper which remains stiff after processing and
> will hold tiny fragments of material, e.g. bone marrow aspirates.
> The paper is made for wrapping small samples and I'm sure other
> people sell similar material.
>
> 1. Cut the end off a plastic dropping pipette.
> 2. Suck up the tissue fragments.
> 3. SLOWLY drop the sample onto the biowrap paper which is placed onto
> a dowmflow bench or a wad of absorbant material. The excess fluid
> will be drawn away and leave the sample in a small spot. The slower
> you do this the smaller the spot will be.
> 4. Wrap up the paper and process.
> 5. At embedding, unwrap the paper and make sure the wax on it remains
> molten.
> 6. Keep a mold hot and put in the paper specimen-side-down.
> 7. Keep the mold hot until the wax is melted both above and below the
> paper, add a little wax to the mold at this stage.
> 8. This is the hard bit. When melted, quickly transfer the mould and
> paper to the cold plate and press the paper into the mould with one
> finger. AS SOON as the wax begins to set, peel the paper off the
> mould and, hey presto, all the tiny bits of tissue are stuck to the
> mould and not the paper.
> 9. Add more wax to the mould and remelt. Allow the bits to settle to
> the bottom on the mould and then set the block ready for sectioning.
> 10. Make sure the microtome chuck is perfectly aligned or you you
> will trim away the sample. For a tip on aligning the mircotome chuck
> see my homepage on histonet.
>
> Best Wishes
> Steve Machin UK
>
> ____________________________________________________________
> Do You Yahoo!?
> Get your free @yahoo.co.uk address at http://mail.yahoo.co.uk
> or your free @yahoo.ie address at http://mail.yahoo.ie
>
----------------------------------------------------------------------
Date: 6 Aug 2001 06:22:57 -0500
From: "Dr. Ian Montgomery" <ian.montgomery@bio.gla.ac.uk>
Subject: What is it?
Just been given a Shandon 2L Processor MkII and as usual it didn't
come with the instruction manual. The instrument is fully functional and I
have spare processor cards and beakers. Although the operation looks clear
I have one wee problem. In the position before the specimen basket a heavy
six-spoked weight hangs from the lid. What in the name of the wee man is
the thing for. Apart from annoying me I can't come up with a sensible
solution. Please help, if you know let me know, or better, if Shandon is
monitoring Histonet what is it.
Ian.
Dr. Ian Montgomery,
Microscopy Service Unit,
Graham Kerr Building,
Institute of Biological & Life Sciences,
University of Glasgow,
Glasgow,
G12 8QQ.
Tel: 0141 332 8855 Extn.6644.
e-mail: ian.montgomery@bio.gla.ac.uk
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<html>
<x-tab> </x-tab>Just been
given a Shandon 2L Processor MkII and as usual it didn't come with the
instruction manual. The instrument is fully functional and I have spare
processor cards and beakers. Although the operation looks clear I have
one wee problem. In the position before the specimen basket a heavy
six-spoked weight hangs from the lid. What in the name of the wee man is
the thing for. Apart from annoying me I can't come up with a sensible
solution. Please help, if you know let me know, or better, if Shandon is
monitoring Histonet what is it.<br>
Ian. <br>
<br>
<br>
<x-sigsep><p></x-sigsep>
<font color="#0000FF">Dr. Ian Montgomery,<br>
Microscopy Service Unit,<br>
Graham Kerr Building,<br>
Institute of Biological & Life Sciences,<br>
University of Glasgow,<br>
Glasgow,<br>
G12 8QQ.<br>
Tel: 0141 332 8855 Extn.6644.<br>
e-mail: ian.montgomery@bio.gla.ac.uk</font></html>
- --=====================_2090422==_.ALT--
----------------------------------------------------------------------
Date: 6 Aug 2001 06:23:15 -0500
From: "Dr. Ian Montgomery" <ian.montgomery@bio.gla.ac.uk>
Subject: Fwd: textbooks
Aidan,
Histological & Histochemical Methods. Theory and Practice. Third Edition
1999.
J.A.Kiernan.
Butterworth-Heineman. ISBN 0 7506 3106 6
This is a superb text and should be on every Histotechnologists
book shelf. Apart from a source of techniques it's one of those books you
can simply browse again and again for the wealth of information it holds.
(Sorry for the blushes John)
Ian.
>Date: Mon, 06 Aug 2001 16:12:14 +1200
>From: Aidan Schurr <Aidan.Schurr@hvh.co.nz>
>Subject: textbooks
>To: histonet@pathology.swmed.edu
>
>Hi all,
>
>I'm preparing a "wish-list" for the year. Can anyone recommend a good,
>new textbook (a "must-have" if you will). I have a copy of the latest
>edition of Bancroft already. I tried to source a new edition of Sheehan
>and Hrapchak, but it appears this has been out of print since 1987!
>
>Thanks for all your comments in advance.
>
>Cheers,
>Aidan
>
>
>__
>
>aidan schurr b.m.l.sc
>section head, histology
>hutt valley district health board
>lower hutt
>new zealand
>
>aidan.schurr@hvh.co.nz
>++64 4 570 9173
Dr. Ian Montgomery,
Microscopy Service Unit,
Graham Kerr Building,
Institute of Biological & Life Sciences,
University of Glasgow,
Glasgow,
G12 8QQ.
Tel: 0141 332 8855 Extn.6644.
e-mail: ian.montgomery@bio.gla.ac.uk
******************* NOTE *******************
There may be important message content
contained in the following MIME Information.
********************************************
- ------------------ MIME Information follows ------------------
- --=====================_2238067==_.ALT
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<html>
Aidan,<br>
<b>Histological & Histochemical Methods. </b>Theory and Practice.
Third Edition 1999.<br>
J.A.Kiernan.<br>
Butterworth-Heineman. ISBN 0 7506 3106 6 <br>
<br>
<x-tab> </x-tab>This is a
superb text and should be on every Histotechnologists book shelf. Apart
from a source of techniques it's one of those books you can simply browse
again and again for the wealth of information it holds. <br>
(Sorry for the blushes John) <br>
Ian.<br>
<br>
<br>
<br>
<br>
<br>
<br>
<br>
<blockquote type=cite class=cite cite>Date: Mon, 06 Aug 2001 16:12:14
+1200<br>
From: Aidan Schurr <Aidan.Schurr@hvh.co.nz><br>
Subject: textbooks<br>
To: histonet@pathology.swmed.edu<br>
<br>
Hi all,<br>
<br>
I'm preparing a "wish-list" for the year. Can anyone
recommend a good, new textbook (a "must-have" if you
will). I have a copy of the latest edition of Bancroft
already. I tried to source a new edition of Sheehan and Hrapchak,
but it appears this has been out of print since 1987!<br>
<br>
Thanks for all your comments in advance.<br>
<br>
Cheers,<br>
Aidan<br>
<br>
<br>
__<br>
<br>
aidan schurr b.m.l.sc<br>
section head, histology<br>
hutt valley district health board<br>
lower hutt<br>
new zealand<br>
<br>
aidan.schurr@hvh.co.nz<br>
++64 4 570 9173</blockquote>
<x-sigsep><p></x-sigsep>
<font color="#0000FF">Dr. Ian Montgomery,<br>
Microscopy Service Unit,<br>
Graham Kerr Building,<br>
Institute of Biological & Life Sciences,<br>
University of Glasgow,<br>
Glasgow,<br>
G12 8QQ.<br>
Tel: 0141 332 8855 Extn.6644.<br>
e-mail: ian.montgomery@bio.gla.ac.uk</font></html>
- --=====================_2238067==_.ALT--
----------------------------------------------------------------------
Date: 6 Aug 2001 07:41:08 -0500
From: T.Hacker@har.mrc.ac.uk
Subject: Re: What is it?
Date sent: Mon, 06 Aug 2001 12:02:51 +0100
From: "Dr. Ian Montgomery" <ian.montgomery@bio.gla.ac.uk>
Subject: What is it?
To: histonet@pathology.swmed.edu
> I have one wee problem. In the position before the specimen basket a heavy
> six-spoked weight hangs from the lid.
The wee problem could be prostatitis.
The weight is a solid wax detector.
Terry.
----------------------------------------------------------------------
Date: 6 Aug 2001 08:01:01 -0500
From: "Morken, Tim" <tim9@cdc.gov>
Subject: RE: Cryostat cleaning
Kim,
Leica sells a spray-reagent that is supposed to disinfect the cryostat while
still cold. However, I have never seen any documentation that it works.
Documented procedures that work all involve defrosting the cryostat.
Most of these methods are modifications of those used to disinfect
laboratory freezers and the literature is full of those methods.
1) 95% alcohol. This was primarily shown to be effective for HIV, but is
probably effective against most other organisms. It involves wiping down the
interior of the instrument with 95% alcohol. You can take out just the knife
holder and knife and disinfect those.
Reference: "Frozen section technique for tissues infected by the AIDS
virus". Swisher, BL, and Ewing, EP., Journal of Histotechnology, Vol 9,
No.1, March 1996.
2) 100 percent alcohol is recommended by NACCLS, but they don't give any
documention to support their recommendation. This is also recommended by
Woods and Ellis in their book "Laboratory Histopathology", chap 2, pg 19.
3) Formalin vapor. This involves derfrosting the cryostat and placing a
container of 37% formalin inside overnight. In studies this effectivly kills
organisms in petri dishes. (Shandon, unpublished results. Shandon includes a
formalin method with all their cryostats). Note that this method will not
work in a cold cryostat as the formlin loses its effctiveness at freezing
temperatures.
4) To disinfect for TB you need to use a specialty TB disinfectant to
satisfy your safety officer. Check with them for what type they recommend.
Again, defrosting is necessary.
One way to avoid defrosting is to use a vacuum attachment to suck up debris
before it can conaminate the interior of the cabinet. They you only need to
clean the knife and/or knife holder. I'm sure a certain company out there in
HistoLand can tell you all about that vacuum.
Tim Morken EMT(MSA), HTL(ASCP)
Centers for Disease Control and Prevention
Infectious Disease Pathology
MS-G32
1600 Clifton Road
Atlanta, GA 30333
PH 404-639-3964
FAX 404-639-3043
email tim9@cdc.gov
- -----Original Message-----
From: Brady, Kimberly [mailto:kbrady@eosbiotech.com]
Sent: Friday, August 03, 2001 4:00 PM
To: 'histonet@pathology.swmed.edu'
Subject:
Does anyone know of a good disinfectant that can be used to clean a cryostat
while it is cold? I work in a lab that uses one cryostat to cut both mouse
and human tissue (we just started cutting human tissue) and our cleaning
protocol after cutting human tissue is to turn off the cryostat and to use
commercial bleach. Unfortunately, some parts look like they are starting to
rust, and it is very time consuming to let the cryostat warm up for
cleaning, then to cool down again for use. I am new at histology (4 months)
and I am open to any advice anyone has for me...
Thanks,
Kim
----------------------------------------------------------------------
Date: 6 Aug 2001 08:01:17 -0500
From: "Dr. Ian Montgomery" <ian.montgomery@bio.gla.ac.uk>
Subject: What is it?
Terry and Mike,
Many thanks, problem solved. Seems very sensible so I'll
away and dangle the spoke in its position. See all this modern technology,
fair smartens you up a bit.
Ian.
Dr. Ian Montgomery,
Microscopy Service Unit,
Graham Kerr Building,
Institute of Biological & Life Sciences,
University of Glasgow,
Glasgow,
G12 8QQ.
Tel: 0141 332 8855 Extn.6644.
e-mail: ian.montgomery@bio.gla.ac.uk
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Terry and Mike,<br>
<x-tab> </x-tab><x-tab>
</x-tab>Many
thanks, problem solved. Seems very sensible so I'll away and dangle the
spoke in its position. See all this modern technology, fair smartens you
up a bit.<br>
Ian.<br>
<x-sigsep><p></x-sigsep>
<font color="#0000FF">Dr. Ian Montgomery,<br>
Microscopy Service Unit,<br>
Graham Kerr Building,<br>
Institute of Biological & Life Sciences,<br>
University of Glasgow,<br>
Glasgow,<br>
G12 8QQ.<br>
Tel: 0141 332 8855 Extn.6644.<br>
e-mail: ian.montgomery@bio.gla.ac.uk</font></html>
- --=====================_7176958==_.ALT--
----------------------------------------------------------------------
Date: 6 Aug 2001 08:46:23 -0500
From: Heather Diamond <diamondrecruiter@yahoo.com>
Subject: new positions
Morning everyone,
www.hdsrjobs.com has been updated over the weekend to
include the following new positions in:
Nebraska
North Dakota
Kansas
Boulder
Dorchester, Mass
San Diego
Bradenton, Florida
Dallas, Texas
Panama City, FL
Palm Beach, FL
Portland, OR
Thanks,
Heather Diamond
HDSR
Sr. Recruiter
www.hdsrjobs.com
Toll Free 866-258-1406
__________________________________________________
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Make international calls for as low as $.04/minute with Yahoo! Messenger
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----------------------------------------------------------------------
Date: 6 Aug 2001 08:46:38 -0500
From: JHoffpa464@aol.com
Subject: Re: Sentinel Lymph Node Biopsy Specimen Handling
You might check out the following website. <A
HREF="http://www.uic.com.au/nip26.htm">Radioisotopes in Medicine</A>
john
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<HTML><FONT FACE=arial,helvetica><FONT SIZE=2>You might check out the
following website. <A HREF="http://www.uic.com.au/nip26.htm">Radioisotopes
in
Medicine</A>
<BR>john</FONT></HTML>
- --part1_8e.196316e7.289ff0c5_boundary--
----------------------------------------------------------------------
Date: 6 Aug 2001 10:16:24 -0500
From: Cynthia Favara <cfavara@niaid.nih.gov>
Subject: test
I am getting notification of enclosures and I am not sending any this is a
test again.
Cynthia Favara
Rocky Mountain Laboratories
903 S. 4th Street
Hamilton, MT 59840
406-363-9317
FAX 406-363-9286
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<P><FONT SIZE=2>I am getting notification of enclosures and I am not sending
any this is a test again.</FONT>
<BR><FONT SIZE=2>Cynthia Favara</FONT>
<BR><FONT SIZE=2>Rocky Mountain Laboratories</FONT>
<BR><FONT SIZE=2>903 S. 4th Street</FONT>
<BR><FONT SIZE=2>Hamilton, MT 59840</FONT>
<BR><FONT SIZE=2>406-363-9317</FONT>
<BR><FONT SIZE=2>FAX 406-363-9286</FONT>
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----------------------------------------------------------------------
Date: 6 Aug 2001 10:39:39 -0500
From: Dave Low <lowman034@yahoo.com>
Subject: Re: Paraffin embedding tiny bits o' tissue
Hi Rachel,
The small aluminum implement is a tissue tamper (I had
to think myself!). This product is availble through:
Sakura Finetek U.S.A., Inc
1750 West 214th Street
Torrance, CA 90501
Tel: 800-725-8723
Fax: 310-972-7888
The tampers come in two sizes, a small and large.
Contact the company for the catalogy number, I know
I had to pay around $20 for each package. Good luck!
D.Low HT(ASCP)QIHC
Elmendorf Med Ctr, AK
- --- Rachel Munshower <rachelm@anatomy.iupui.edu>
wrote:
> Does anybody use a small aluminum implement (I can't
> remember the name) to
> keep your tissue flush with your bottom edge of the
> paraffin block?
> Sometimes when I put a rat's heart and lung is a
> block, one organ is
> sitting "behind" the other one and it's makin me
> crazy. ANybody know what
> it's called and where I can get one?
> Many Thanks!
> Rachel
>
> ________________________________________
> Rachel Munshower
> IU School of Medicine
> Dept. of Anatomy and Cell Biology
> 635 Barnhill Dr. MedSci 5065
> Indy, IN 46202
> 317-274-2505
>
>
>
__________________________________________________
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----------------------------------------------------------------------
Date: 6 Aug 2001 11:07:32 -0500
From: Kathie Berghorn <kab35@cornell.edu>
Subject: Overheating cut paraffin slides?
Dear Histonetters,
I am relatively new at using paraffin sections -- I had a
student cutting paraffin sections over the weekend and to 'help'
sections dry on the slide warmer after cutting, turned up the slide
warmer enough that it melted the paraffin around the tissue.
My question is.... is the tissue still usable? Did the heat
damage proteins within the tissue affecting the antigenicity?
Thanking you in advance for your replies,
Kathie
----------------------------------------------------------------------
Date: 6 Aug 2001 11:37:22 -0500
From: Heather Diamond <diamondrecruiter@yahoo.com>
Subject: North Carolina Histology Supervisor
Hello,
Just in!
Needed: HISTOLOGY SUPERVISOR located North Carolina
near VA/NC borderline, is currently seeking
applications for the position of Histology Supervisor.
Candidates should have a minimum of four years
experience as a Histology Technician and HTL(ASCP)
registry or five years experience and HT(ASCP)
registry.
Let me know if you'd like to be considered.
Thanks,
Heather Diamond
HDSR
Sr. Recruiter
www.hdsrjobs.com
__________________________________________________
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----------------------------------------------------------------------
Date: 6 Aug 2001 11:51:40 -0500
From: Jaclynn Lett <jlett@cid.wustl.edu>
Subject: clearing agents
We're looking for clearing agents for viewing whole mounts of mouse cochleas
under the dissecting scope. They'll be labeled with fluorescent tags such
as Cy-3, Texas Red and ethidium homodimers, so the clearing agent must not
dissolve the tag. Also, since we'll be looking at the tissue under a scope,
the agent should not be toxic nor have obnoxious fumes.
I'm aware of the citrus-based clearing agents, and oils such as wintergreen
(methyl salicylate), but have no idea as to they're suitability for our
purpose (or whether the cochleas should be decalcified as well).
If anyone has any insight, I would much appreciated the assistance.
Thank you,
Jaclynn M. Lett, Research Technician
Fay and Carl Simons Center for Biology of Hearing and Deafness
Central Institute for the Deaf
4560 Clayton Avenue
St. Louis, MO 63110
jlett@cid.wustl.edu
----------------------------------------------------------------------
Date: 6 Aug 2001 12:36:18 -0500
From: "Morken, Tim" <tim9@cdc.gov>
Subject: RE: textbooks
Two new ones that are good are:
Antigen Retrieval Techniques; by Shi, Gu and Taylor; Eaton Publishing
Biotechniques Books Division (www.biotechniques.com). all the details you'd
ever want to know about antigen retrieval.
and
Immunoenzyme Multiple Staining Methods, by C.M. van der Loos; Royal
Microscopy Society, BIOS Scientific Publishers, thru Springer-Verlag.
(www.springer-ny.com). Essential for anyone doing multiple immunostaining.
Tim Morken EMT(MSA), HTL(ASCP)
Centers for Disease Control and Prevention
Infectious Disease Pathology
MS-G32
1600 Clifton Road
Atlanta, GA 30333
PH 404-639-3964
FAX 404-639-3043
email tim9@cdc.gov
- -----Original Message-----
From: Aidan Schurr [mailto:Aidan.Schurr@hvh.co.nz]
Sent: Monday, August 06, 2001 12:12 AM
To: histonet@pathology.swmed.edu
Subject: textbooks
Hi all,
I'm preparing a "wish-list" for the year. Can anyone recommend a good, new
textbook (a "must-have" if you will). I have a copy of the latest edition
of Bancroft already. I tried to source a new edition of Sheehan and
Hrapchak, but it appears this has been out of print since 1987!
Thanks for all your comments in advance.
Cheers,
Aidan
__
aidan schurr b.m.l.sc
section head, histology
hutt valley district health board
lower hutt
new zealand
aidan.schurr@hvh.co.nz
++64 4 570 9173
----------------------------------------------------------------------
Date: 6 Aug 2001 12:36:38 -0500
From: Mary Thiel <MThiel@lexgen.com>
Subject: unsubsubscribe
***************************************************************************
The contents of this communication are intended only for the addressee and
may contain confidential and/or privileged material. If you are not the
intended recipient, please do not read, copy, use or disclose this
communication and notify the sender. Opinions, conclusions and other
information in this communication that do not relate to the official
business of my company shall be understood as neither given nor endorsed by
it.
***************************************************************************
----------------------------------------------------------------------
Date: 6 Aug 2001 13:04:33 -0500
From: nlgervais@mmm.com
Subject: Specimen Retention Policy
I am asking this question on behalf of a colleague. Responses may be sent
to TheisenK@centracare.com.
We are considering adding this statement to our Surgical Consent Form:
All specimens, including tissue, foreign bodies, and prosthetics, delivered
to the histology/pathology department will undergo, at a minimum, a gross
examination. The pathologist will perform microscopic exam of specimens if
requested or deemed necessary. Specimens and explanted prosthetic devices
are retained for one month after the pathology report has been generated or
returned to the patient upon request within that time period. Thereafter
if the patient or doctor has not requested their return or extended
storage, the explanted devices will be discarded.
Should we proceed or should we just address retention practices in our
department policies?
Thank you in advance.
----------------------------------------------------------------------
Date: 6 Aug 2001 13:25:21 -0500
From: "Connolly, Brett M" <brett_connolly@merck.com>
Subject: VEGF in rat
All,
I'm looking for an anti-rat VEGF antibody for paraffin embedded
tissue...mono or poly. Any suggestions?
Brett
Brett M. Connolly, Ph.D.
Merck Research Laboratories
Dept. of Pharmacology
WP26A-3000
PO Box 4
West Point, PA 19486
PH 215-652-2501
fax. 215-652-2075
e-mail. brett_connolly@merck.com
----------------------------------------------------------------------
Date: 6 Aug 2001 13:25:37 -0500
From: Todd Sherman <t-sherman@home.com>
Subject: Re: Overheating cut paraffin slides?
Hello Kathie,
Having the paraffin melt around the tissue is usually not a problem;
however,
the effect of heat on protein antigenicity
can be determined empirically only. I've performed experiments where
melting
the paraffin was quite acceptable and
other experiments where a particular epitope (or the antibody-antigen
binding
complex) was "destroyed" - well, at least
modified enough such that the antibody could not bind well enough to create
an
observable signal.
If the student's tissue block is not completely used up, could you not
produce
more sections and not warm them as
much (or at all)? This could be a useful pursuit in illustrating the
concept
of optimizing experimental conditions. By
adding a few slides to the study you might be able to demonstrate the effect
of changing one variable in your
procedure. The result could be "no significant effect", "significantly
positive effect", or "significantly negative effect"
and teach some valuable lessons in experimental procedure.
At any rate, good luck with your study and let us know your results.
Regards,
Todd Sherman
t-sherman@home.com
8/6/2001 10:21:40 AM, Kathie Berghorn <kab35@cornell.edu> wrote:
>Dear Histonetters,
> I am relatively new at using paraffin sections -- I had a
>student cutting paraffin sections over the weekend and to 'help'
>sections dry on the slide warmer after cutting, turned up the slide
>warmer enough that it melted the paraffin around the tissue.
>
> My question is.... is the tissue still usable? Did the heat
>damage proteins within the tissue affecting the antigenicity?
>
> Thanking you in advance for your replies,
> Kathie
----------------------------------------------------------------------
Date: 6 Aug 2001 14:12:29 -0500
From: Histonet <Histonet@dakousa.com>
Subject: RE: Slide labelling
Dear Histonet,
Dear Histonet,
DAKO redesigned the Seymour labels due to customer feedback. Here are the
improvements that were implemented that enhance the performance of the
labels when exposed to xylene and heat.
* The Seymour Label adhesive was improved to withstand longer exposure to
xylene, especially after heat treatment. This is a dramatic improvement over
the previous formulation, and we have virtually eliminated adhesive
dissolving from the labels.
* The label stock was improved to provide a brighter label that is more
stain resistant to typical stains used in IHC, Special Stains and ISH. This
will allow the labels to be easy to read, and look clean.
If you plan to soak the slides in xylene for extended amounts of time (In
excess of 30 minutes), lowering the level of xylene so that the label is not
directly exposed will improve the performance of the labels.
Sincerely,
Danielle Mach
Technical Service Specialist
DAKO Corporation
800-424-0021
- -----Original Message-----
From: Kimberly Carter [mailto:carter.343@osu.edu]
Sent: Tuesday, July 31, 2001 6:40 AM
Cc: 'Histonet'
Subject: Re: Slide labelling
I have been using the DAKO labeling system for 2 years. One thing you need
to be
aware of is that although the writing on the label is resistant to solvents,
the
glue on the back of the label is not. You have to keep your levels of
solutions
low enough not to come into contact with the label, especially any xylene
(This
includes longer exposure to xylene fumes also). The glue becomes goo and
will
seep out and down the slide. Otherwise, I agree with Tim Morken's accessment
of
the label maker.
Kim Carter
OSU Medical Center
Comprehensive Cancer Center
Columbus, Ohio
"Morken, Tim" wrote:
> The DAKO Seymour slide label system would meet your needs perfectly. It
can
> be installed on any Windows computer. The printer and software is from
DAKO.
> The labels are thermal-printed and then covered with a plastic flap. They
> are totally resistant to any histology lab solutions and solvents. The
only
> drawback is that each label must be typed into the system, although there
is
> some flexibility for repeating labels and lines.
>
> This system is cheaper than the slide etching systems, although those are
> very good also (we have both).
>
> Tim Morken
> CDC, Atlanta
>
> -----Original Message-----
> From: Coaker, Terry [mailto:Terry.Coaker@nuth.northy.nhs.uk]
> Sent: Tuesday, July 31, 2001 6:00 AM
> To: 'Histonet'
> Subject: Slide labelling
>
> Does anyone use slide labels that are resistant to solvents and stains,
that
> can be computer generated, and affixed to the slide at the waterbath ? I
> would like to eliminate the slide labelling step after staining (time
> consuming and a potential source of error)
>
> Thankyou
>
> Terry Coaker
>
> Chief Biomedical Scientist
> Cellular Pathology
> Royal Victoria Infirmary
> Newcastle upon Tyne NE1 4LP
> terry.coaker@nuth.northy.nhs.uk
> 0191 282 4445
>
----------------------------------------------------------------------
Date: 6 Aug 2001 14:13:41 -0500
From: "PMarcum" <pmarcum@polysciences.com>
Subject: RE: Alcian Blue 8GX
Sirs,
Has the Alcian Blue 8GX been certified or tested through the Biological
Stain Commission? If it has a BSC number we would be interested.
Pamela A. Marcum
Histology/Microscopy
Product Development Manager
400 Valley Road
Warrington, PA 18976
Phone: 800-523-2575 Ext 167
215-343-6484 Ext 167
Fax: 215-343-0214
E-mail: pmarcum@polysciences.com
- -----Original Message-----
From: Sinoera Tech [mailto:billions@public1.sz.js.cn]
Sent: Saturday, August 04, 2001 3:52 AM
To: HistoNet Server
Subject: Re: Alcian Blue 8GX
We are research based custom manufactuer in line of biological stains,
dyes, indicators, we may serve the histology field with our efforts, we can
supply Alcian blue 8GX, and Alcian yellow is on the reseach way.
Kind Regards
Suzhou Sinoera Chem Co., Ltd.
Fax: 0086 512 8224995, 8258994
Tel: 0086 512 8246939
e-mail: billions@public1.sz.js.cn
----------------------------------------------------------------------
Date: 6 Aug 2001 14:14:00 -0500
From: "PMarcum" <pmarcum@polysciences.com>
Subject: RE: fat analysis
The Vibratome may be your best bet if you don't want to freeze the tissue.
It can be very frustrating to cut with the Vibratome however it does allow
the use of slightly fixed to well fixed tissue to be cut in thicker
sections. The tissue can also be kept very cold but not frozen. It just
does not produce even sections. They often look like venetian blinds.
Pam Marcum
- -----Original Message-----
From: emaher9@home.com [mailto:emaher9@home.com]
Sent: Saturday, August 04, 2001 2:12 AM
To: Robert Geske; histonet@pathology.swmed.edu
Subject: Re: fat analysis
How about trying a vibrating microtome? I don't know if the fat is solid
enough to try this, but thick sections of brain are cut this way.
Evanne Maher
Leica Specimen Preparation
- ----- Original Message -----
From: "Robert Geske" <RGeske@lexgen.com>
To: <histonet@pathology.swmed.edu>
Sent: Saturday, July 28, 2001 5:47 AM
Subject: fat analysis
> we are currently engaged in quantitation of different types and anatomic
> locations of fat. we want to analyze the adipocytes in terms of their
number
> per unit volume and the individual cells maximum diameter and from that
> infer cell volume. given that the cells maximum diameter can be up to 200
> microns, we want to sample slices of fat in excess of that number.
> we initially looked at 200 micron sections of fat frozen in OCT and
> sectioned in a cryostat. this was not adequate. our cryostat will
section
> up to 300 microns in thickness and this is the next step. the sections
are
> floated on a buffer and viewed at 4X with images being captured with a
> digital camera before analysis. we do not want to mount the sections on
> slides as this causes shape distortion (this was our first thought
also ---
> section, mount, and ORO). are there any suggestions on equipment and/or
> techniques for cutting up to 500 micron sections of fixed or fresh fat.
> another possiblity i am looking into this weekend is sterological methods
to
> determine the information we are after. we this, i believe, we will not
> have to take such thick sections. with the knowledge of the measurment of
> section thickness and area we should be able to analyze multiple planes
> through the section and make reasonable estimates about the population of
> adipocytes. does anyone have experience using this method for fat
analysis?
>
> regards to all,
>
> rob
>
>
***************************************************************************
> The contents of this communication are intended only for the addressee
and
> may contain confidential and/or privileged material. If you are not the
> intended recipient, please do not read, copy, use or disclose this
> communication and notify the sender. Opinions, conclusions and other
> information in this communication that do not relate to the official
> business of my company shall be understood as neither given nor endorsed
by
> it.
>
***************************************************************************
>
>
>
----------------------------------------------------------------------
Date: 6 Aug 2001 14:14:14 -0500
From: "THERESA ROHR" <trohratnyackhospital@hotmail.com>
Subject: CK 5/6
Hi!
I am again seeking help with a "new" antibody for my lab. Is anyone using
DAKO CK 5/6 currently with Envision Plus on the Autostainer?
If so, what dilution is working well for you? Also, what tissue would you
suggest as a good control?
It is great having access to your "minds" and also hearing from DAKO Reps.
This site is saving me a lot of time and money by narrowing down my dilution
testing both in terms of time and reagents.
Thanks in advance,
Theresa Rohr BA HT(ASCP)
Nyack Hospital
New York
_________________________________________________________________
Get your FREE download of MSN Explorer at http://explorer.msn.com/intl.asp
----------------------------------------------------------------------
Date: 6 Aug 2001 15:21:23 -0500
From: "Laurie Colbert" <laurie.colbert@schs.com>
Subject: Histoplasmosis-decontamination of cryostat
We have a cryostat that is contaminated with histoplasmosis. What is
the best way to decontaminate - bleach? alcohol?
Laurie Colbert
Huntington Memorial Hospital
Pasadena, CA
----------------------------------------------------------------------
Date: 6 Aug 2001 15:21:38 -0500
From: mward@wfubmc.edu (Martha Ward)
Subject: RCC antibody
Martha Ward wrote:
I have just had a request for RCC (renal cell carcinoma) antibody and I
wondered if anyone has any experience with this on a Ventana ES. I need
vendor/ conditions, etc. Thanks in advance for your help!
----------------------------------------------------------------------
Date: 6 Aug 2001 15:22:00 -0500
From: "Barry Rittman" <brittman@mail.db.uth.tmc.edu>
Subject: Re: Overheating cut paraffin slides?
Kathie
the answer is maybe.
It is difficult to tell, as it depends on the temperature, time of
exposure, the proteins in question and how dry the section was before
the paraffin was melted. We used to routinely heat paraffin sections of
hard tissue but only after the section appeared dry to the eye. This
helped in section adhesion of the bone and dentin to the slide. With
these there did not seem to be any changes in staining or
immunochemistry compared to "non melted" sections.
I think you will just have to try it and see for the specific proteins
you are interested in. I would also run some other slides that have not
been heated as extra controls to make sure that you have the specificity
that you would normally expect.
Barry
Kathie Berghorn wrote:
> Dear Histonetters,
> I am relatively new at using paraffin sections -- I had a
> student cutting paraffin sections over the weekend and to 'help'
> sections dry on the slide warmer after cutting, turned up the slide
> warmer enough that it melted the paraffin around the tissue.
>
> My question is.... is the tissue still usable? Did the heat
> damage proteins within the tissue affecting the antigenicity?
>
> Thanking you in advance for your replies,
> Kathie
----------------------------------------------------------------------
Date: 6 Aug 2001 15:22:16 -0500
From: "Morken, Tim" <tim9@cdc.gov>
Subject: RE: Specimen Retention Policy
If it's going on a consent form you better have the lawyers look at it
first!
Tim Morken
Atlanta
- -----Original Message-----
From: nlgervais@mmm.com [mailto:nlgervais@mmm.com]
Sent: Monday, August 06, 2001 1:20 PM
To: histonet@pathology.swmed.edu
Subject: Specimen Retention Policy
I am asking this question on behalf of a colleague. Responses may be sent
to TheisenK@centracare.com.
We are considering adding this statement to our Surgical Consent Form:
All specimens, including tissue, foreign bodies, and prosthetics, delivered
to the histology/pathology department will undergo, at a minimum, a gross
examination. The pathologist will perform microscopic exam of specimens if
requested or deemed necessary. Specimens and explanted prosthetic devices
are retained for one month after the pathology report has been generated or
returned to the patient upon request within that time period. Thereafter
if the patient or doctor has not requested their return or extended
storage, the explanted devices will be discarded.
Should we proceed or should we just address retention practices in our
department policies?
Thank you in advance.
----------------------------------------------------------------------
Date: 6 Aug 2001 15:25:59 -0500
From: "Joan Roach" <jlmroach@hotmail.com>
Subject: Stieve's Fixative
Hi
Does anyone know the composition of Stieve's fixative? And its uses?
Thanks
Joan
_________________________________________________________________
Get your FREE download of MSN Explorer at http://explorer.msn.com/intl.asp
----------------------------------------------------------------------
Date: 6 Aug 2001 16:45:18 -0500
From: RSRICHMOND@aol.com
Subject: Re: Alcian Blue 8GX
Pamela A. Marcum in Warrington, Pennsylvania, asks:
>>Has the Alcian Blue 8GX [offered by Suzhou Sinoera Chem Co., Ltd.] been
certified or tested through the Biological Stain Commission? If it has a BSC
number we would be interested.<<
An even more fundamental question: is the dye synthesis being done (this is
in Soochow, China, now - formerly in India and Pakistan) with due regard to
the safety of the workers in handling the apparently very hazardous
materials
used in the old-time secret synthetic procedure for Alcian blue?
I have no connection with Anatech, but I'll toot their horn for them:
they're
offering Alcian blue which they describe as having been synthesized by an
environmentally safe synthetic pathway.
Apparently neither side has had any luck with Alcian yellow.
Bob Richmond
Samurai Pathologist
Knoxville, Tennessee USA
----------------------------------------------------------------------
Date: 6 Aug 2001 16:45:39 -0500
From: Jkpresnell2@cs.com
Subject: Attention! ALL NSH ATTENDEES!
Attention!!!
All NSH Attendees
We still need a few volunteers for Liaisons for the following workshops
#1 #3 #7 # 22 #24 #25 #27 #29 #31 #32 #39 #43 #59 #60 #67
#70 #71 #73 #74 #92 #98 #99.
If it is "computer" workshop, you still have to pay the computer fee, $40.
Remember, you have to attend the Liaison Meeting on Friday at 6:00PM on
Friday.
Please consult the registration form for additional details.
If you are already signed up to be a volunteer, Please Email me or call/fax
at 865-573-4708. You can also communicate with Sylvia at the NSH office.
We
need to finish the final assignments as soon as possible.
Thank you,
Janice Presnell
NSH workroom
Assistant Manager
jkpresnell2@cs.com
----------------------------------------------------------------------
Date: 6 Aug 2001 16:46:02 -0500
From: "Vinnie Della Speranza" <dellav@musc.edu>
Subject: RE: Specimen Retention Policy
I agree with Tim. Doubtful that this list's members are knowledgeable as to
what will hold up in a court of law. Your facility's attorneys need to bless
it.
Vinnie Della Speranza
Manager for Anatomic Pathology Services
Medical University of South Carolina
165 Ashley Avenue Suite 309
Charleston, SC 29425
Ph: 843-792-6353
fax: 843-792-8974
>>> "Morken, Tim" <tim9@cdc.gov> 08/06/01 02:46PM >>>
If it's going on a consent form you better have the lawyers look at it
first!
Tim Morken
Atlanta
- -----Original Message-----
From: nlgervais@mmm.com [mailto:nlgervais@mmm.com]
Sent: Monday, August 06, 2001 1:20 PM
To: histonet@pathology.swmed.edu
Subject: Specimen Retention Policy
I am asking this question on behalf of a colleague. Responses may be sent
to TheisenK@centracare.com.
We are considering adding this statement to our Surgical Consent Form:
All specimens, including tissue, foreign bodies, and prosthetics, delivered
to the histology/pathology department will undergo, at a minimum, a gross
examination. The pathologist will perform microscopic exam of specimens if
requested or deemed necessary. Specimens and explanted prosthetic devices
are retained for one month after the pathology report has been generated or
returned to the patient upon request within that time period. Thereafter
if the patient or doctor has not requested their return or extended
storage, the explanted devices will be discarded.
Should we proceed or should we just address retention practices in our
department policies?
Thank you in advance.
----------------------------------------------------------------------
Date: 6 Aug 2001 18:45:42 -0500
From: DDittus787@aol.com
Subject: Re: CK 5/6
We do not use dako stainer or antibody, we use ventana stainer and Zymed
antibody, however Basal Call carcinoma is a great control and we enzyme
before staining. Dana
******************* NOTE *******************
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contained in the following MIME Information.
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- --part1_e4.18ef7ecc.28a0831c_boundary
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<HTML><FONT FACE=arial,helvetica><FONT SIZE=2>We do not use dako stainer or
antibody, we use ventana stainer and Zymed
<BR>antibody, however Basal Call carcinoma is a great control and we enzyme
<BR>before staining.
&nbs
p; &n
bsp;
Dana</FONT></HTML>
- --part1_e4.18ef7ecc.28a0831c_boundary--
----------------------------------------------------------------------
Date: 6 Aug 2001 19:01:53 -0500
From: Andrea Grantham <algranth@u.arizona.edu>
Subject: Sirius Red/Fast Blue
Well it is Monday and somebody just walked in the door asking for something
new - at least to me - a collagen stain using Sirius Red and Fast Blue.
I've looked through all my textbooks and find nothing - has anyone heard of
this? They have a great picture but no procedure.
Andi Grantham
.....................................................................
: Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy :
: Sr. Research Specialist University of Arizona :
: (office: AHSC 4212) P.O. Box 245044 :
: (voice: 520-626-4415) Tucson, AZ 85724-5044 USA :
: (FAX: 520-626-2097) (email: algranth@u.arizona.edu) :
:...................................................................:
http://www.cba.arizona.edu/histology-lab.html
----------------------------------------------------------------------
Date: 6 Aug 2001 19:54:08 -0500
From: "Lee & Peggy Wenk" <lpwenk@mail.netquest.com>
Subject: Re: Stieve's Fixative
I've seen it mentioned with IHC procedures, usually as being
superior to formalin fixation.
I tried to look it up once, but only found a brief reference that it
was a formol sublimate fixative. Since that means it contains
formaldehyde and mercuric chloride salt, I assumed it would
be similar to B-5 fixative, and didn't investigate anymore.
Sorry I can't be more helpful.
Peggy A. Wenk, HTL(ASCP)
William Beaumont Hospital
Royal Oak, MI 48073
- ----- Original Message -----
From: "Joan Roach" <jlmroach@hotmail.com>
To: <histonet@pathology.swmed.edu>
Cc: <NWarner@usc.edu>
Sent: Monday, August 06, 2001 3:01 PM
Subject: Stieve's Fixative
> Hi
> Does anyone know the composition of Stieve's fixative? And its uses?
> Thanks
> Joan
>
> _________________________________________________________________
> Get your FREE download of MSN Explorer at http://explorer.msn.com/intl.asp
>
>
>
----------------------------------------------------------------------
Date: 6 Aug 2001 21:13:31 -0500
From: kbowden@ucsd.edu
Subject: Re: clearing agents
We have used methyl salicylate on a vascular study. We used india ink as
our
marker. It worked very well. I don't know if it would fade or remove
fluorecent dyes. If you need a protocol let me know and I'll send it to
you.
- -- ************************
Karen Bowden
kbowden@ucsd.edu
Research Associate
Department of Orthopedics - 0630
University of California, San Diego
9500 Gilman Dr.
La Jolla, CA 92093-0630
(858) 277-4970
(858) 277-4974 Fax
Jaclynn Lett wrote:
> We're looking for clearing agents for viewing whole mounts of mouse
cochleas
> under the dissecting scope. They'll be labeled with fluorescent tags such
> as Cy-3, Texas Red and ethidium homodimers, so the clearing agent must not
> dissolve the tag. Also, since we'll be looking at the tissue under a
scope,
> the agent should not be toxic nor have obnoxious fumes.
>
> I'm aware of the citrus-based clearing agents, and oils such as
wintergreen
> (methyl salicylate), but have no idea as to they're suitability for our
> purpose (or whether the cochleas should be decalcified as well).
>
> If anyone has any insight, I would much appreciated the assistance.
>
> Thank you,
>
> Jaclynn M. Lett, Research Technician
>
> Fay and Carl Simons Center for Biology of Hearing and Deafness
> Central Institute for the Deaf
> 4560 Clayton Avenue
> St. Louis, MO 63110
>
> jlett@cid.wustl.edu
----------------------------------------------------------------------
Date: 6 Aug 2001 21:52:23 -0500
From: "jamie dukes" <jduk22@hotmail.com>
Subject: freida carson text
<html><div style='background-color:#996699'><H1>Does anyone have a Freida
Carson textbook that is collecting dust I would be glad to take it off your
hand.I am willing to pay for it.</H1></div><br clear=all><hr>Get your FREE
download of MSN Explorer at <a
href='http://go.msn.com/bql/hmtag_itl_EN.asp'>http://explorer.msn.com</a><br
></html>
----------------------------------------------------------------------
Date: 6 Aug 2001 22:49:29 -0500
From: "Sinoera Tech" <billions@public1.sz.js.cn>
Subject: Re: Alcian Blue 8GX
Suzhou Sinoera Chem Co., Ltd. (it is located in Suzhou, China) now is
making this product in bulk by a new way,
different from ICI's, and is certified as a safe and environment way
approved by Government, we have idea to do this for 5 years if the HISTOLOGY
applications require it.
We will soon push the Alcian Yellow, Alcian Green into production in bulk,
not labs job.
Minggeng Wang, Ph.D
Suzhou Sinoera Chem Co., Ltd.
- ----- Original Message -----
From: <RSRICHMOND@aol.com>
To: <>
Sent: Tuesday, August 07, 2001 5:01 AM
Subject: Re: Alcian Blue 8GX
| Pamela A. Marcum in Warrington, Pennsylvania, asks:
|
| >>Has the Alcian Blue 8GX [offered by Suzhou Sinoera Chem Co., Ltd.] been
| certified or tested through the Biological Stain Commission? If it has a
BSC
| number we would be interested.<<
|
| An even more fundamental question: is the dye synthesis being done (this
is
| in Soochow, China, now - formerly in India and Pakistan) with due regard
to
| the safety of the workers in handling the apparently very hazardous
materials
| used in the old-time secret synthetic procedure for Alcian blue?
|
| I have no connection with Anatech, but I'll toot their horn for them:
they're
| offering Alcian blue which they describe as having been synthesized by an
| environmentally safe synthetic pathway.
|
| Apparently neither side has had any luck with Alcian yellow.
|
| Bob Richmond
| Samurai Pathologist
| Knoxville, Tennessee USA
----------------------------------------------------------------------
Date: 7 Aug 2001 00:04:56 -0500
From: "J. A. Kiernan" <jkiernan@uwo.ca>
Subject: Re: clearing agents
On Mon, 6 Aug 2001, Jaclynn Lett wrote:
> We're looking for clearing agents for viewing whole mounts of mouse
cochleas
> under the dissecting scope. They'll be labeled with fluorescent tags such
> as ...
Try benzyl benzoate. It's optically better than meth sal, and
doesn't smell awful like methyl benzoate (which is optically
best, with refractive index closest to
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