staining prostate biopsies
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Everyone---Thanks for all the renal help. Our system is now up and going with
great success. I was wondering if I could approach you all with another
problem? This same pathologist has brought us a business of numerous
prostate biopsies. we process these on a short cycle---cut at 5 microns--and
stain on Leica stainers using the Richard Allan sytem of stains--Hematoxylin
2--4.5 min clarifier 2--1 min --bluing--30 sec. The pathologist is
complaining that he is unable to see the nuclei and is having extreme
difficulty telling CA patients without the use of a high molecular weight
keratin. All routine surgicals stain the same way only for 4 min hem--are
fine. Prostates stained at 4 are too lite. He suggested we use Harleco
Hematoxylin instead of Richard Allen. I am reluctant to do this beause the
Richard Allen system has been successful so far and the company itself is
great on stain QC. Although I have not had a chance to work thru some various
staining times, I thought that somone may have a suggestion or two out there.
Thanks for your help
Angie Schmidt----Tri-Health
Labs---Cincinnati, Ohio
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<HTML><FONT FACE=arial,helvetica><FONT COLOR="#000080" SIZE=2 FAMILY="SCRIPT" FACE="Comic Sans MS" LANG="0">Everyone---Thanks for all the renal help. Our system is now up and going with
<BR>great success. I was wondering if I could approach you all with another
<BR>problem? This same pathologist has brought us a business of numerous
<BR>prostate biopsies. we process these on a short cycle---cut at 5 microns--and
<BR>stain on Leica stainers using the Richard Allan sytem of stains--Hematoxylin
<BR>2--4.5 min clarifier 2--1 min --bluing--30 sec. The pathologist is
<BR>complaining that he is unable to see the nuclei and is having extreme
<BR>difficulty telling CA patients without the use of a high molecular weight
<BR>keratin. All routine surgicals stain the same way only for 4 min hem--are
<BR>fine. Prostates stained at 4 are too lite. He suggested we use Harleco
<BR>Hematoxylin instead of Richard Allen. I am reluctant to do this beause the
<BR>Richard Allen system has been successful so far and the company itself is
<BR>great on stain QC. Although I have not had a chance to work thru some various
<BR>staining times, I thought that somone may have a suggestion or two out there.
<BR> Thanks for your help
<BR> Angie Schmidt----Tri-Health
<BR>Labs---Cincinnati,
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