staining prostate biopsies

From:Schmang155@aol.com


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Everyone---Thanks for all the renal help. Our system is now up and going with 
great success. I was wondering if I could approach you all with another 
problem? This same pathologist  has brought us a business of numerous 
prostate biopsies. we process these on a short cycle---cut at 5 microns--and 
stain on Leica stainers using the Richard Allan sytem of stains--Hematoxylin 
2--4.5 min clarifier 2--1 min --bluing--30 sec.   The pathologist is 
complaining that he is unable to see the nuclei and is having extreme 
difficulty telling CA patients without the use of a high molecular weight 
keratin. All routine surgicals stain the same way only for 4 min hem--are 
fine. Prostates stained at 4 are too lite. He suggested we use Harleco 
Hematoxylin instead of Richard Allen. I am reluctant to do this beause the 
Richard Allen system has been successful so far and the company itself is 
great on stain QC. Although I have not had a chance to work thru some various 
staining times, I thought that somone may have a suggestion or two out there. 
 Thanks for your help
                                      Angie Schmidt----Tri-Health 
Labs---Cincinnati, Ohio

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<HTML><FONT FACE=arial,helvetica><FONT  COLOR="#000080" SIZE=2 FAMILY="SCRIPT" FACE="Comic Sans MS" LANG="0">Everyone---Thanks for all the renal help. Our system is now up and going with 
<BR>great success. I was wondering if I could approach you all with another 
<BR>problem? This same pathologist  has brought us a business of numerous 
<BR>prostate biopsies. we process these on a short cycle---cut at 5 microns--and 
<BR>stain on Leica stainers using the Richard Allan sytem of stains--Hematoxylin 
<BR>2--4.5 min clarifier 2--1 min --bluing--30 sec.   The pathologist is 
<BR>complaining that he is unable to see the nuclei and is having extreme 
<BR>difficulty telling CA patients without the use of a high molecular weight 
<BR>keratin. All routine surgicals stain the same way only for 4 min hem--are 
<BR>fine. Prostates stained at 4 are too lite. He suggested we use Harleco 
<BR>Hematoxylin instead of Richard Allen. I am reluctant to do this beause the 
<BR>Richard Allen system has been successful so far and the company itself is 
<BR>great on stain QC. Although I have not had a chance to work thru some various 
<BR>staining times, I thought that somone may have a suggestion or two out there. 
<BR> Thanks for your help
<BR>                                      Angie Schmidt----Tri-Health 
<BR>Labs---Cincinnati, 

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