re: tissue processing times for bovine/porcine samples
|From:||Louri Caldwell <firstname.lastname@example.org>|
I apologize for the first post - I hit the "send" button a moment too soon!
Our routine processing schedule is as follows. All are done under
pressure/vacuum, with the reagents at 37 and paraffin at 60.
Formalin 30 minutes
Alcoholic Formalin 2 changes, 45 minutes each
80% Ethanol 1 hour
95% Ethanol 2 changes, 1 hour each
100% Ethanol 2 changes, 1 hour each
Xylene 2 changes, 1 hour each
Paraffin 3 changes, 2 at 50 minutes, 1 at 30
Hope this helps.
College of Veterinary Medicine
University of Georgia
>From: "Goodwin, Sylene" <Sylene.Goodwin@smbio.com>
>Subject: re: tissue processing times for bovine/porcine samples
>Date: Mon, 13 Aug 2001 12:30:57 -0500
> Our histology group has been asked to process some steer and pig
>samples for paraffin embedding. We normally do rat tissue....could any of
>the Vet/Large Animal Histopaths give me some ideas as to processing times
>an automated processing machine?
>Sylene MJ Goodwin
>Sulzer Biologics Inc.
>4056 Youngfield Street
>Wheat Ridge, CO 80033
>Tel: 303.467.9766 x181
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