re: tissue processing times for bovine/porcine samples

From:Louri Caldwell <louri_c@hotmail.com>

I apologize for the first post - I hit the "send" button a moment too soon!

Our routine processing schedule is as follows.  All are done under 
pressure/vacuum, with the reagents at 37 and paraffin at 60.

Formalin             30 minutes
Alcoholic Formalin   2 changes, 45 minutes each
80% Ethanol          1 hour
95% Ethanol          2 changes, 1 hour each
100% Ethanol         2 changes, 1 hour each
Xylene               2 changes, 1 hour each
Paraffin             3 changes, 2 at 50 minutes, 1 at 30

Hope this helps.

Louri Caldwell
Chief Histotechnologist
College of Veterinary Medicine
University of Georgia
Athens, Georgia

>From: "Goodwin, Sylene" <Sylene.Goodwin@smbio.com>
>To: histonet@pathology.swmed.edu
>Subject: re: tissue processing times for bovine/porcine samples
>Date: Mon, 13 Aug 2001 12:30:57 -0500
>
>Good Morning;
>
>	Our histology group has been asked to process some steer and pig
>samples for paraffin embedding.  We normally do rat tissue....could any of
>the Vet/Large Animal Histopaths give me some ideas as to processing times 
>on
>an automated processing machine?
>
>thank-you!!
>
>Sylene MJ Goodwin
>
>SULZERMEDICA
>Sulzer Biologics Inc.
>4056 Youngfield Street
>Wheat Ridge, CO 80033
>
>Tel:  303.467.9766  x181
>Fax: 303.467.1982
>sylene.goodwin@smbio.com
>
>


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