mouse on mouse immunos biotinylated antibodies

From:Gayle Callis <uvsgc@montana.edu>

If this works with that particular antibody.   We buy the primary antibody
already biotinylated, actually have many of our murine antibodies in that
form to begin with, and have few cross reaction problems.    

At 08:04 AM 8/3/01 -0600, you wrote:
>
>----- Original Message -----
>From: "Jan Shivers" <shive003@tc.umn.edu>
>To: "Harvey, Jennifer" <Jennifer_Harvey@URMC.Rochester.edu>
>Sent: Thursday, August 02, 2001 3:52 PM
>Subject: Re: mouse on mouse immunos
>
>
>> Try a DAKO ARK kit.  It biotinylates your primary antibody directly and
>> avoids that crossreactivity one normally gets when using a biotinylated
>> anti-mouse IgG linking antibody.
>>
>> Jan Shivers
>> Univ of Minnesota Vet Diag Lab
>>
>> ----- Original Message -----
>> From: "Harvey, Jennifer" <Jennifer_Harvey@URMC.Rochester.edu>
>> To: "'Histonet'" <histonet@pathology.swmed.edu>
>> Sent: Thursday, August 02, 2001 11:35 AM
>> Subject: mouse on mouse immunos
>>
>>
>> >
>> >
>> > Histonet,
>> >
>> > I have had several request in the last few weeks about staining mouse
>> > tissues with mouse monoclonal antibodies. I have problems with this but
>I
>> > have heard that it can work. What is your opinions and if you say it can
>> > work how do you do it?
>> >
>> > Jennifer Harvey HT (ASCP), QIHC
>> > University of Rochester
>> > Department of Orthopaedics
>> > Rochester, NY
>> >
>> >
>> >
>> >
>> >
>>
>
>
>
>
Gayle Callis
MT,HT,HTL(ASCP)
Histopathology Supervisor
Veterinary Molecular Biology
Montana State Univer

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