decalcified human bone-processing schedule

From:"Marshall, Sharon," <>

Hi histonetters,
I am processing small bits (to fit a histo. cassette) of human bone 
which have tendon attached. I have decalcified in 2% nitric acid. (
Checked endpoint.) I would like some advice on the best way to 
process these pieces of bone plus attached tendon. I do not have much 
experience working with bone samples and I have obtained a lot of 
information off the histonet.  I feel that I should extend processing 
times but I would like to know what is optimal without wasting too 
much time. 
Sharon Marshall

<< Previous Message | Next Message >>