decalcified human bone-processing schedule
From: | "Marshall, Sharon," <marshall@cormack.uct.ac.za> |
Hi histonetters,
I am processing small bits (to fit a histo. cassette) of human bone
which have tendon attached. I have decalcified in 2% nitric acid. (
Checked endpoint.) I would like some advice on the best way to
process these pieces of bone plus attached tendon. I do not have much
experience working with bone samples and I have obtained a lot of
information off the histonet. I feel that I should extend processing
times but I would like to know what is optimal without wasting too
much time.
Thanks
Sharon Marshall
e-mail:
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