background in FFPE spleen

From:"Philopena, Jennifer" <>

Hi.  I'm desperate for suggestions.  Please HELP!
I'm doing IHC on FFPE nude mouse spleen, and I'm having a BIG background
issue.  I do antigen retrieval with citrate buffer, 3% peroxidase quench for
10 minutes, protein block - 30min, rabbit polyclonal antibody - 60min,
biotin goat anti-rabbit secondary - 30min, SA-HRP - 15min, AEC-12min,
Any suggestions?
Thanks a lot.

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