Requesting information on VEGF ANtibody

From:Patricia Bourne <>

Greetings everyone.....I have been working with VEGF
and finding problems with getting our Pathologists to
agree on a method.  On our control, kidney (both fetal
and adult normal) and colon the staining seems fine
with PK retrieval but went I run our colon project
there is even staining or no staining at all.  I ran
the antibody with high ph before we settled on PK and
it was felt that too much was staining.  Now the
question is most catalogs that have VEGF say High ph
so I was hoping that someone out there has a method
that was working consistently and could share some
insite ....what should be staining, is this antigen
sensitive fixation or oxidation, what titer are you
using with what retrieval method, where are you
getting your antibody ( ours is from Santa Cruz), what
tissues have you used with your method ....I know its
alot of questions but the articles I have read are not
helping me much and I need to move along with this
project...thanks for your help in advance. 

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