Re: mouse on mouse immunos

From:James Mubiru (by way of histonet)

I have used the Mouse on Mouse (MOM) Vector kit and it
worked out fine with me althuogh I could still see
some unspecific staining in and around the blood
vessels, but it was not a big deal. I used it to stain
for cytokeratin 8/18 in mouse prostate using a mouse
monoclonal. I do not know if I had not used the MOM
system the results would have been the same. One thing
I realized with the Vector MOM kit is that they  do
not say in which animal the second antibody is raised
James Mubiru

--- "Harvey, Jennifer"
> Histonet,
> I have had several request in the last few weeks
> about staining mouse
> tissues with mouse monoclonal antibodies. I have
> problems with this but I
> have heard that it can work. What is your opinions
> and if you say it can
> work how do you do it?
> Jennifer Harvey HT (ASCP), QIHC
> University of Rochester
> Department of Orthopaedics
> Rochester, NY

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