Re: clearing agents
|From:||Karen Bowden <firstname.lastname@example.org>|
We have used methyl salicylate on a vascular study. We used india ink as our
marker. It worked very well. I don't know if it would fade or remove
fluorecent dyes. If you need a protocol let me know and I'll send it to you.
Department of Orthopedics - 0630
University of California, San Diego
9500 Gilman Dr.
La Jolla, CA 92093-0630
(858) 277-4974 Fax
Jaclynn Lett wrote:
> We're looking for clearing agents for viewing whole mounts of mouse cochleas
> under the dissecting scope. They'll be labeled with fluorescent tags such
> as Cy-3, Texas Red and ethidium homodimers, so the clearing agent must not
> dissolve the tag. Also, since we'll be looking at the tissue under a scope,
> the agent should not be toxic nor have obnoxious fumes.
> I'm aware of the citrus-based clearing agents, and oils such as wintergreen
> (methyl salicylate), but have no idea as to they're suitability for our
> purpose (or whether the cochleas should be decalcified as well).
> If anyone has any insight, I would much appreciated the assistance.
> Thank you,
> Jaclynn M. Lett, Research Technician
> Fay and Carl Simons Center for Biology of Hearing and Deafness
> Central Institute for the Deaf
> 4560 Clayton Av
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