Re: chrome-alum gelatin

From:"J. A. Kiernan" <>

On Tue, 31 Jul 2001, Sunil Thomas K wrote:

> Is there any rationale in thinking that the adhesion
> would be better if the concentration is increased?

It allows you to make a thicker coating on the slide.
I have done this for a particularly harsh method
(benzil in alkaline alcohol, to block arginine, followed
by an alkaline aqueous reagent) and it reduced section
losses. For that job, thick chrome-gelatin was better
than APES, thin chrome-gelatin or starch paste.
John A. Kiernan
Department of Anatomy & Cell Biology
The University of Western Ontario
London,  Canada   N6A 5C1

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