<br><font size=2 face="Courier New">-- "Bartlett, Jeanine" <jqb7@cdc.gov> wrote:<br>
> All:<br>
> <br>
> I need some help. I am trying to find an aqueous<br>
> mountant that I can use on<br>
> an IHC that has DAKO Fast Red as the substrate and<br>
> uses methyl green as a<br>
> counterstain instead of Mayer's hematoxylin. The<br>
> DAKO Faramount pulls all<br>
> of the green out of the sections. Does anyone have<br>
> any ideas?<br>
> <br>
> Thanks!<br>
> Jeanine Bartlett, HT(ASCP)<br>
> Centers for Disease Control<br>
> Infectious Disease Pathology Activity<br>
> Atlanta, GA 30333 </font>
<br>
<br><font size=2 face="sans-serif">Hi Jeanine,</font>
<br>
<br><font size=2 face="sans-serif">We don't use Fast Red nor do we use methyl green so I can't comment on those stains specifically. But, we do use AEC and therefore an aqueous mountant. The mountant that we use is Aquaperm which we get from Fisher Scientific. The product no. is 28-610-54. We apply it to the wet slides and then solidify the mountant in a 70 degree Celcius oven for approximately 30 minutes. We then coverslip the slides routinely with xylene, resinous mountant and coverslips. I hope this helps. Tom </font>
<br>
<br><font size=2 face="sans-serif">Tom Wells BSc, ART</font>
<br><font size=2 face="sans-serif">Immunohistochemistry Laboratory</font>
<br><font size=2 face="sans-serif">Lions Gate Hospital</font>
<br><font size=2 face="sans-serif">North Vancouver, BC</font>
<br><font size=2 face="sans-serif">Canada</font>