Re: Sirius Red/Fast Blue
From: | RichardWHorobin@aol.com |
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Andi writes:
> Well it is Monday and somebody just walked in the door asking for something
> new - at least to me - a collagen stain using Sirius Red and Fast Blue.
> I've looked through all my textbooks and find nothing - has anyone heard of
> .
Noting John Kiernan's comments re dye identity, I wonder if the blue dye was
LUXOL fast blue? Salthouse did come up with stains using this, both for
collagen and elastin staining, way back. Try looking at:
Salthouse TN. 1965. Selective staining of collagen and elastic by luxol fast
blue G in methanol. J Histochem Cytochem. 13: 133-140
Salthouse TN. 1966. Preferential staining of collagen by acid dyes in
methanol. Nature (London). 210: 1277.
And do let me know if they work for you
Bye now - RIchard Horobin
Institute of Biomedical & Life Sciences, University of Glasgow
T direct 01796-474 480 --- E RichardWHorobin@aol.com
"What should we expect? Everything."
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<HTML><FONT FACE=arial,helvetica><FONT SIZE=2>Andi writes:
<BR>
<BR><BLOCKQUOTE TYPE=CITE style="BORDER-LEFT: #0000ff 2px solid; MARGIN-LEFT: 5px; MARGIN-RIGHT: 0px; PADDING-LEFT: 5px">Well it is Monday and somebody just walked in the door asking for something
<BR>new - at least to me - a collagen stain using Sirius Red and Fast Blue.
<BR>I've looked through all my textbooks and find nothing - has anyone heard of
<BR>this? They have a great picture but no procedure</BLOCKQUOTE>.</FONT><FONT COLOR="#000000" SIZE=3 FAMILY="SANSSERIF" FACE="Arial" LANG="0">
<BR>
<BR>Noting John Kiernan's comments re dye identity, I wonder if the blue dye was
<BR>LUXOL fast blue? Salthouse did come up with stains using this, both for
<BR>collagen and elastin staining, way back. Try looking at:
<BR>
<BR>Salthouse TN. 1965. Selective staining of collagen and elastic by luxol fast
<BR>blue G in methanol. J Histochem Cytochem. 13: 133-140
<BR>
<BR>Salthouse TN. 1966. Preferential staining of collagen by acid dyes in
<BR>methanol. Nature (London). 210: 1277.
<BR>
<BR>And do let me know if they work for you
<BR>Bye now - RIchard Horobin
<BR>
<BR></FONT><FONT COLOR="#000000" SIZE=2 FAMILY="SANSSERIF" FACE="Arial" LANG="0">Institute of Biomedical & Life Sciences, University of Glasgow
<BR><B>T direct 01796-474 480 --- E RichardWHorobin@aol.com</B>
<BR><I>"What should we expect?
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