Re: RE Cyclin-D1

From:DDittus787@aol.com


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I use Zymed Cyclin D1, on the nexes, however we microwave retrieve on high 
for 5 min 30 sec. then on 30%(defrost mode)power for 11 min 30 sec. stain for 
32 min. As long as control tissue is relatively fresh( less than 2 weeks if 
stored at room temp,)good staining. Good Luck.              Dana

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<HTML><FONT FACE=arial,helvetica><FONT  SIZE=2>I use Zymed Cyclin D1, on the nexes, however we microwave retrieve on high 
<BR>for 5 min 30 sec. then on 30%(defrost mode)power for 11 min 30 sec. stain for 
<BR>32 min. As long as control tissue is relatively fresh( less than 2 weeks if 
<BR>stored at room temp,)good staining. Good Luck.              Dana</FONT></HTML>

--pa

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