Re: Quality-right first time?
Date sent: Thu, 09 Aug 2001 09:06:56 +0100
From: "Coaker, Terry" <Terry.Coaker@nuth.northy.nhs.uk>
Subject: Quality-right first time?
To: "'Histonet'" <email@example.com>
> What is the most reliable, economical way of ensuring section quality
> with no transcription errors or recuts?
> Is it necessary to check every slide microscopically before issue?
I would say yes to the latter question. In an ideal world we would
produce the perfect section first time, every time, but this is not
always possible or obvious until viewed microscopically. I actively
encourage my staff to use the microscope to assess their work,
this gives them a better knowledge of Histology and Pathology (and
makes my life easier).Ultimately I look at all the slides as I have to
report or discuss most of them, but have found that the number of
recuts/rejects have become less and less.
As for economy, what is the cost of checking slides
microscopically. This should be considered an invaluable teaching
Transcription errors/section quality, do you mean putting the wrong
name/number on the slide?
> staff are trained to pick up only the best sections at the water bath
> and you control your staining, can you be confident in the quality and
> remove microscopic checking?
No, this may not pick up any of those artefacts associated with
fixation, processing etc which need to be identified rapidly or
indeed staining problems.
Remember the pathologists provide the
> final check.
Yes, but I am sure their time is better suited to reporting rather
than requesting recuts. Better you sort these things out before the
work gets to the Pathologist.
> Are there other ways e.g checking all slides off against the paraffin
Is this not standard procedure in quality control?
> or using a stereo microscope to check quality at the water bath.
This will give some indication of section quality/level etc. but this
would not really be adequate in the final analysis.
As with much in Histology, results can be very subjective as could
ones perception of quality, although there is not much room for
Head of Histopathology and Electron Microscopy
Medical Research Council,
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