Re: LCM for paraffin sections

From:Kimberly Carter <>

  In your response to the LCM issue, you said to put the tissue on adhesive
coated slides. I am curious if  anyone at your company tried this in an
actual LCM procedure? (Or have first hand knowledge of anyone) If so, I
would like to try it. Please forward any info on this issue. All of my
information and experience says to use uncoated plain slides. Since with
this procedure you don't capture an image, but  pull the tissue off of the
slide with a laser, then do DNA and/or RNA analysis on the tissue, using
adhesive slides are not recommended. I want the least amount of tissue
bonding to the slide as possible and while still staying on the slide. As
for your paraffin tape-transfer system, I am interested in trying this
product. The water bath step, as with all the steps in this procedure, is
tedious and time consuming. As far as the section being as thin as desired,
I want 8-10u sections. Does the tape work well at this thickness also? And
can the tape transfer tissue onto plain slides? Thanks for the suggestions
you have sent. Preparing the slides for LCM is one of my least favorite
tasks in the lab. Any improvements in this procedure would be welcomed!

Kim Carter
OSU Medical Center
Comprehensive Cancer Center
Columbus, Ohio

"Instrumedics, Inc." wrote:

> Instrumedics'  Paraffin Tape-Transfer system can be very helpful in
> preparing a paraffin section for laser capture microdissection(LCM). The
> tape adhered to the block face supports and captures the section which
> can
> be as thin as desired.
> The water bath step is eliminated!
> The section is transferred directly to an adhesive coated slide. After
> the
> adhesive is cured the tape is removed.  The section is anchored to the
> slide.
> Section loss is no longer a problem! The area of interest can then be
> captured!
> Precautions are taken in the preparation of the slides for LCM to insure
> they are RNAse free. During the cutting and staining procedures care must
> be
> taken not to introduce any contaminants. Kim Carter discussed her
> procedure.
> Bernice

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