Re: LCM for paraffin sections
|From:||Kimberly Carter <email@example.com>|
In your response to the LCM issue, you said to put the tissue on adhesive
coated slides. I am curious if anyone at your company tried this in an actual
LCM procedure? (Or have first hand knowledge of anyone) If so, I would like to
try it. Please forward any info on this issue. All of my information and
experience says to use uncoated plain slides. Since with this procedure you
don't capture an image, but pull the tissue off of the slide with a laser, then
do DNA and/or RNA analysis on the tissue, using adhesive slides are not
recommended. I want the least amount of tissue bonding to the slide as possible
and while still staying on the slide. As for your paraffin tape-transfer system,
I am interested in trying this product. The water bath step, as with all the
steps in this procedure, is tedious and time consuming. As far as the section
being as thin as desired, I want 8-10u sections. Does the tape work well at this
thickness also? And can the tape transfer tissue onto plain slides? Thanks for
the suggestions you have sent. Preparing the slides for LCM is one of my least
favorite tasks in the lab. Any improvements in this procedure would be welcomed!
OSU Medical Center
Comprehensive Cancer Center
"Instrumedics, Inc." wrote:
> Instrumedics' Paraffin Tape-Transfer system can be very helpful in
> preparing a paraffin section for laser capture microdissection(LCM). The
> tape adhered to the block face supports and captures the section which can
> be as thin as desired.
> The water bath step is eliminated!
> The section is transferred directly to an adhesive coated slide. After the
> adhesive is cured the tape is removed. The section is anchored to the
> Section loss is no longer a problem! The area of interest can then be
> Precautions are taken in the preparation of the slides for LCM to insure
> they are RNAse free. During the cutting and staining procedures care must be
> taken not to introduce any contaminants. Kim Carter discussed her procedure.
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