Hi, Gerard,
We got great staining from the One-step and it is easy to do.  There are
less steps and while slides were incubating in the solutions I could be
doing something else.  And as was mentioned I did microwave the Bouins
step -- 2.5min on the "warm" setting and then 20min in 60C oven.

Nancy Weber
Research Associate
Veterinary Clinical Sciences
Ohio State University
----- Original Message -----
From: "Gerard Spoelstra" <spoelstr@numbat.murdoch.edu.au>
To: <histonet@pathology.swmed.edu>
Sent: Saturday, August 11, 2001 4:18 AM
Subject: Re: Gomoris one step trichrome


> Dear Nancy, the comment in "Manual of Histological techniques by Bancroft
> and Cook,1984 is that this stain is used for frozen sections and not
> paraffin. If you have paraffin sections why not use a Massons?
> Gerard Spoelstra
> Medical Scientist
> School of Veterinary and Biomedical Science
> Murdoch University
> Western Australia
>
>
> At 10:13 PM 10/08/01 -0400, you wrote:
> >Ian,  I agree with Nancy. The One Step is a good stain and we use it
often.
> >We did shorten the procedure by microwaving 20 mls of the Bouin's for
> >10-15 seconds.  Then we place the slides in the small staining container
> >with the Bouin's in a 60C oven for 10-15 minutes.  Still looks great but
> >in a few less minutes.  Best of Luck
> >
> >Histologically yours,
> >Barbara H. Stancel
> >USDA, FSIS, OPHS, Eastern Laboratory, Pathology
> >RRC, 950 College Station Road
> >Athens, Georgia  30604
> >phone: (706) 546-3556
> >fax: (706) 546-3589
> >
> >
> >
> >>From: Nancy E Weber <weber.326@osu.edu>
> >>To: Clarke Ian <clarke.ian@virgin.net>
> >>CC: histonet <histonet@pathology.swmed.edu>
> >>Subject: Re: Re- Gomoris one step trichrome
> >>Date: Thu, 09 Aug 2001 09:09:10 -0400
> >>
> >>Hi Ian,
> >>
> >>Here's the Gomori's One Step,  And this is the method that Victor
Tobias,
> >>that Gayle mentioned,  would have put on the net.
> >>
> >>1. Deparaffinize and hydrate to distilled water.
> >>2. Place in Bouin's solution in oven at 60C for one hour.
> >>3. Wash will in running tap water till yellow color is gone.
> >>4. Rinse in distilled water.
> >>5. Stain in Weigert's hematoxylin for 10min.
> >>6.  Wash in running tap water for 10 min
> >>7. Place in Gomori's trichrome stain for 20 min
> >>8. Differentiate in 0.5% glacial acetic acid for 2 min
> >>9. Dehydrate, clear, and mount
> >>
> >>Muscle fibers.........................Red
> >>Collagen..............................Green
> >>Nuclei..............................Blue to Black
> >>
> >>Weigert's Hematoxylin
> >>      Soln A
> >>          Hematoxylin ..............................1.0 gm
> >>          95% alcohol..............................100.0 ml
> >>      Soln B
> >>          Ferric Chloride 29% aqueous.........4.0 ml
> >>          Distilled Water..............................95.0 ml
> >>          Hydrochloric Acid,  concentrated......1.0 ml
> >>Weigert's Working ... Mix equal parts of soln A and
> >>soln B.
> >>
> >>Gomori's Trichrome
> >>     Chromotrope 2R.............................0.6 gm
> >>     Light Green, SF yellowish................0.3 gm
> >>     Glacial acetic acid........................... 1.0 ml
> >>     Phosphotungstic acid........................0.8 gm
> >>     Distilled Water...............................100 ml
> >>
> >>Reference:  Luna, L.G.  1968, Manual of Histologic Staining Methods of
the
> >>Armed Forces Institiute of Pathology,  (3rd Ed.) (pp 93)
> >>
> >>Nancy Weber
> >>Research Associate
> >>Veterinary Clinical Sciences
> >>Ohio State University
> >>----- Original Message -----
> >>From: "Clarke Ian" <clarke.ian@virgin.net>
> >>To: "histonet" <histonet@pathology.swmed.edu>
> >>Sent: Wednesday, August 08, 2001 4:39 PM
> >>Subject: Re- Gomoris one step trichrome
> >>
> >>
> >> > Hi all ,
> >> > I am looking for the constituents of Gomoris one step Trichrome,is it
> >> > comparable to the Masson Trichrome Technique?
> >> > Thanks in advance
> >> > Ian Clarke
> >> > Craigavon Area Hospital
> >> > N.Ireland
> >> >
> >> >
> >> >
> >>
> >
> >
> >________________________