Dear Nancy, the comment in "Manual of Histological techniques by Bancroft 
and Cook,1984 is that this stain is used for frozen sections and not 
paraffin. If you have paraffin sections why not use a Massons?
Gerard Spoelstra
Medical Scientist
School of Veterinary and Biomedical Science
Murdoch University
Western Australia


At 10:13 PM 10/08/01 -0400, you wrote:
>Ian,  I agree with Nancy. The One Step is a good stain and we use it often.
>We did shorten the procedure by microwaving 20 mls of the Bouin's for 
>10-15 seconds.  Then we place the slides in the small staining container 
>with the Bouin's in a 60C oven for 10-15 minutes.  Still looks great but 
>in a few less minutes.  Best of Luck
>
>Histologically yours,
>Barbara H. Stancel
>USDA, FSIS, OPHS, Eastern Laboratory, Pathology
>RRC, 950 College Station Road
>Athens, Georgia  30604
>phone: (706) 546-3556
>fax: (706) 546-3589
>
>
>
>>From: Nancy E Weber <weber.326@osu.edu>
>>To: Clarke Ian <clarke.ian@virgin.net>
>>CC: histonet <histonet@pathology.swmed.edu>
>>Subject: Re: Re- Gomoris one step trichrome
>>Date: Thu, 09 Aug 2001 09:09:10 -0400
>>
>>Hi Ian,
>>
>>Here's the Gomori's One Step,  And this is the method that Victor Tobias,
>>that Gayle mentioned,  would have put on the net.
>>
>>1. Deparaffinize and hydrate to distilled water.
>>2. Place in Bouin's solution in oven at 60C for one hour.
>>3. Wash will in running tap water till yellow color is gone.
>>4. Rinse in distilled water.
>>5. Stain in Weigert's hematoxylin for 10min.
>>6.  Wash in running tap water for 10 min
>>7. Place in Gomori's trichrome stain for 20 min
>>8. Differentiate in 0.5% glacial acetic acid for 2 min
>>9. Dehydrate, clear, and mount
>>
>>Muscle fibers.........................Red
>>Collagen..............................Green
>>Nuclei..............................Blue to Black
>>
>>Weigert's Hematoxylin
>>      Soln A
>>          Hematoxylin ..............................1.0 gm
>>          95% alcohol..............................100.0 ml
>>      Soln B
>>          Ferric Chloride 29% aqueous.........4.0 ml
>>          Distilled Water..............................95.0 ml
>>          Hydrochloric Acid,  concentrated......1.0 ml
>>Weigert's Working ... Mix equal parts of soln A and
>>soln B.
>>
>>Gomori's Trichrome
>>     Chromotrope 2R.............................0.6 gm
>>     Light Green, SF yellowish................0.3 gm
>>     Glacial acetic acid........................... 1.0 ml
>>     Phosphotungstic acid........................0.8 gm
>>     Distilled Water...............................100 ml
>>
>>Reference:  Luna, L.G.  1968, Manual of Histologic Staining Methods of the
>>Armed Forces Institiute of Pathology,  (3rd Ed.) (pp 93)
>>
>>Nancy Weber
>>Research Associate
>>Veterinary Clinical Sciences
>>Ohio State University
>>----- Original Message -----
>>From: "Clarke Ian" <clarke.ian@virgin.net>
>>To: "histonet" <histonet@pathology.swmed.edu>
>>Sent: Wednesday, August 08, 2001 4:39 PM
>>Subject: Re- Gomoris one step trichrome
>>
>>
>> > Hi all ,
>> > I am looking for the constituents of Gomoris one step Trichrome,is it
>> > comparable to the Masson Trichrome Technique?
>> > Thanks in advance
>> > Ian Clarke
>> > Craigavon Area Hospital
>> > N.Ireland
>> >
>> >
>> >
>>
>
>
>____________________________________